1p4t
From Proteopedia
(New page: 200px<br /><applet load="1p4t" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p4t, resolution 2.55Å" /> '''Crystal structure of...) |
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| - | [[Image:1p4t.jpg|left|200px]]<br /><applet load="1p4t" size=" | + | [[Image:1p4t.jpg|left|200px]]<br /><applet load="1p4t" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1p4t, resolution 2.55Å" /> | caption="1p4t, resolution 2.55Å" /> | ||
'''Crystal structure of Neisserial surface protein A (NspA)'''<br /> | '''Crystal structure of Neisserial surface protein A (NspA)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The neisserial surface protein A (NspA) from Neisseria meningitidis is a | + | The neisserial surface protein A (NspA) from Neisseria meningitidis is a promising vaccine candidate because it is highly conserved among meningococcal strains and induces bactericidal antibodies. NspA is a homolog of the Opa proteins, which mediate adhesion to host cells. Here, we present the crystal structure of NspA, determined to 2.55-A resolution. NspA forms an eight-stranded antiparallel beta-barrel. The four loops at the extracellular side of the NspA molecule form a long cleft, which contains mainly hydrophobic residues and harbors a detergent molecule, suggesting that the protein might function in the binding of hydrophobic ligands, such as lipids. In addition, the structure provides a starting point for structure-based vaccine design. |
==About this Structure== | ==About this Structure== | ||
| - | 1P4T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Neisseria_meningitidis Neisseria meningitidis] with SO4, CXE and ETA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1P4T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Neisseria_meningitidis Neisseria meningitidis] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=CXE:'>CXE</scene> and <scene name='pdbligand=ETA:'>ETA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P4T OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Neisseria meningitidis]] | [[Category: Neisseria meningitidis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bos, M | + | [[Category: Bos, M P.]] |
[[Category: Gros, P.]] | [[Category: Gros, P.]] | ||
[[Category: Tommassen, J.]] | [[Category: Tommassen, J.]] | ||
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[[Category: outer membrane protein]] | [[Category: outer membrane protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:25:06 2008'' |
Revision as of 12:25, 21 February 2008
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Crystal structure of Neisserial surface protein A (NspA)
Overview
The neisserial surface protein A (NspA) from Neisseria meningitidis is a promising vaccine candidate because it is highly conserved among meningococcal strains and induces bactericidal antibodies. NspA is a homolog of the Opa proteins, which mediate adhesion to host cells. Here, we present the crystal structure of NspA, determined to 2.55-A resolution. NspA forms an eight-stranded antiparallel beta-barrel. The four loops at the extracellular side of the NspA molecule form a long cleft, which contains mainly hydrophobic residues and harbors a detergent molecule, suggesting that the protein might function in the binding of hydrophobic ligands, such as lipids. In addition, the structure provides a starting point for structure-based vaccine design.
About this Structure
1P4T is a Single protein structure of sequence from Neisseria meningitidis with , and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of Neisserial surface protein A (NspA), a conserved outer membrane protein with vaccine potential., Vandeputte-Rutten L, Bos MP, Tommassen J, Gros P, J Biol Chem. 2003 Jul 4;278(27):24825-30. Epub 2003 Apr 26. PMID:12716881
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