1h1n
From Proteopedia
(New page: 200px<br /><applet load="1h1n" size="450" color="white" frame="true" align="right" spinBox="true" caption="1h1n, resolution 1.12Å" /> '''ATOMIC RESOLUTION ST...) |
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| - | [[Image:1h1n.jpg|left|200px]]<br /><applet load="1h1n" size=" | + | [[Image:1h1n.jpg|left|200px]]<br /><applet load="1h1n" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1h1n, resolution 1.12Å" /> | caption="1h1n, resolution 1.12Å" /> | ||
'''ATOMIC RESOLUTION STRUCTURE OF THE MAJOR ENDOGLUCANASE FROM THERMOASCUS AURANTIACUS'''<br /> | '''ATOMIC RESOLUTION STRUCTURE OF THE MAJOR ENDOGLUCANASE FROM THERMOASCUS AURANTIACUS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of the major endoglucanase from the thermophilic | + | The crystal structure of the major endoglucanase from the thermophilic fungus Thermoascus aurantiacus was determined by single isomorphous replacement at 1.12A resolution. The full sequence supports the classification of the protein in a subgroup of glycoside hydrolase family 5 for which no structural data are available yet. The active site shows eight critical residues, strictly conserved within family 5. In addition, aromatic residues that line the substrate-binding cleft and that are possibly involved in substrate-binding are identified. A number of residues seem to be conserved among members of the subtype, including a disulphide bridge between Cys212 and Cys249. |
==About this Structure== | ==About this Structure== | ||
| - | 1H1N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermoascus_aurantiacus Thermoascus aurantiacus]. Active as [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] Full crystallographic information is available from [http:// | + | 1H1N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermoascus_aurantiacus Thermoascus aurantiacus]. Active as [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H1N OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thermoascus aurantiacus]] | [[Category: Thermoascus aurantiacus]] | ||
| - | [[Category: Beeumen, J | + | [[Category: Beeumen, J Van.]] |
| - | [[Category: Bhat, M | + | [[Category: Bhat, M K.]] |
| - | [[Category: Petegem, F | + | [[Category: Petegem, F Van.]] |
[[Category: Vandenberghe, I.]] | [[Category: Vandenberghe, I.]] | ||
[[Category: endoglucanase]] | [[Category: endoglucanase]] | ||
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[[Category: thermophilic]] | [[Category: thermophilic]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:56:22 2008'' |
Revision as of 10:56, 21 February 2008
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ATOMIC RESOLUTION STRUCTURE OF THE MAJOR ENDOGLUCANASE FROM THERMOASCUS AURANTIACUS
Overview
The crystal structure of the major endoglucanase from the thermophilic fungus Thermoascus aurantiacus was determined by single isomorphous replacement at 1.12A resolution. The full sequence supports the classification of the protein in a subgroup of glycoside hydrolase family 5 for which no structural data are available yet. The active site shows eight critical residues, strictly conserved within family 5. In addition, aromatic residues that line the substrate-binding cleft and that are possibly involved in substrate-binding are identified. A number of residues seem to be conserved among members of the subtype, including a disulphide bridge between Cys212 and Cys249.
About this Structure
1H1N is a Single protein structure of sequence from Thermoascus aurantiacus. Active as Cellulase, with EC number 3.2.1.4 Full crystallographic information is available from OCA.
Reference
Atomic resolution structure of the major endoglucanase from Thermoascus aurantiacus., Van Petegem F, Vandenberghe I, Bhat MK, Van Beeumen J, Biochem Biophys Res Commun. 2002 Aug 9;296(1):161-6. PMID:12147244
Page seeded by OCA on Thu Feb 21 12:56:22 2008
