1tex
From Proteopedia
(New page: 200px<br /><applet load="1tex" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tex, resolution 2.60Å" /> '''Mycobacterium smegma...) |
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| - | [[Image:1tex.jpg|left|200px]]<br /><applet load="1tex" size=" | + | [[Image:1tex.jpg|left|200px]]<br /><applet load="1tex" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1tex, resolution 2.60Å" /> | caption="1tex, resolution 2.60Å" /> | ||
'''Mycobacterium smegmatis Stf0 Sulfotransferase with Trehalose'''<br /> | '''Mycobacterium smegmatis Stf0 Sulfotransferase with Trehalose'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Sulfolipid-1 (SL-1) is an abundant sulfated glycolipid and potential | + | Sulfolipid-1 (SL-1) is an abundant sulfated glycolipid and potential virulence factor found in Mycobacterium tuberculosis. SL-1 consists of a trehalose-2-sulfate (T2S) disaccharide elaborated with four lipids. We identified and characterized a conserved mycobacterial sulfotransferase, Stf0, which generates the T2S moiety of SL-1. Biochemical studies demonstrated that the enzyme requires unmodified trehalose as substrate and is sensitive to small structural perturbations of the disaccharide. Disruption of stf0 in Mycobacterium smegmatis and M. tuberculosis resulted in the loss of T2S and SL-1 formation, respectively. The structure of Stf0 at a resolution of 2.6 A reveals the molecular basis of trehalose recognition and a unique dimer configuration that encloses the substrate into a bipartite active site. These data provide strong evidence that Stf0 carries out the first committed step in the biosynthesis of SL-1 and establish a system for probing the role of SL-1 in M. tuberculosis infection. |
==About this Structure== | ==About this Structure== | ||
| - | 1TEX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis Mycobacterium smegmatis] with TRE as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1TEX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis Mycobacterium smegmatis] with <scene name='pdbligand=TRE:'>TRE</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TEX OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Mycobacterium smegmatis]] | [[Category: Mycobacterium smegmatis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Akey, D | + | [[Category: Akey, D L.]] |
| - | [[Category: Berger, J | + | [[Category: Berger, J M.]] |
| - | [[Category: Bertozzi, C | + | [[Category: Bertozzi, C R.]] |
| - | [[Category: Leary, J | + | [[Category: Leary, J A.]] |
| - | [[Category: Lee, D | + | [[Category: Lee, D H.]] |
| - | [[Category: Lin, F | + | [[Category: Lin, F L.]] |
| - | [[Category: Mougous, J | + | [[Category: Mougous, J D.]] |
| - | [[Category: Munchel, S | + | [[Category: Munchel, S E.]] |
| - | [[Category: Petzold, C | + | [[Category: Petzold, C J.]] |
| - | [[Category: Pratt, M | + | [[Category: Pratt, M R.]] |
| - | [[Category: Riley, L | + | [[Category: Riley, L W.]] |
| - | [[Category: Senaratne, R | + | [[Category: Senaratne, R H.]] |
[[Category: TRE]] | [[Category: TRE]] | ||
[[Category: mycobacterium]] | [[Category: mycobacterium]] | ||
| Line 33: | Line 33: | ||
[[Category: trehalose-2-sulfate]] | [[Category: trehalose-2-sulfate]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:12:47 2008'' |
Revision as of 13:12, 21 February 2008
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Mycobacterium smegmatis Stf0 Sulfotransferase with Trehalose
Overview
Sulfolipid-1 (SL-1) is an abundant sulfated glycolipid and potential virulence factor found in Mycobacterium tuberculosis. SL-1 consists of a trehalose-2-sulfate (T2S) disaccharide elaborated with four lipids. We identified and characterized a conserved mycobacterial sulfotransferase, Stf0, which generates the T2S moiety of SL-1. Biochemical studies demonstrated that the enzyme requires unmodified trehalose as substrate and is sensitive to small structural perturbations of the disaccharide. Disruption of stf0 in Mycobacterium smegmatis and M. tuberculosis resulted in the loss of T2S and SL-1 formation, respectively. The structure of Stf0 at a resolution of 2.6 A reveals the molecular basis of trehalose recognition and a unique dimer configuration that encloses the substrate into a bipartite active site. These data provide strong evidence that Stf0 carries out the first committed step in the biosynthesis of SL-1 and establish a system for probing the role of SL-1 in M. tuberculosis infection.
About this Structure
1TEX is a Single protein structure of sequence from Mycobacterium smegmatis with as ligand. Full crystallographic information is available from OCA.
Reference
Identification, function and structure of the mycobacterial sulfotransferase that initiates sulfolipid-1 biosynthesis., Mougous JD, Petzold CJ, Senaratne RH, Lee DH, Akey DL, Lin FL, Munchel SE, Pratt MR, Riley LW, Leary JA, Berger JM, Bertozzi CR, Nat Struct Mol Biol. 2004 Aug;11(8):721-9. Epub 2004 Jul 18. PMID:15258569
Page seeded by OCA on Thu Feb 21 15:12:47 2008
Categories: Mycobacterium smegmatis | Single protein | Akey, D L. | Berger, J M. | Bertozzi, C R. | Leary, J A. | Lee, D H. | Lin, F L. | Mougous, J D. | Munchel, S E. | Petzold, C J. | Pratt, M R. | Riley, L W. | Senaratne, R H. | TRE | Mycobacterium | Sulfation | Sulfolipid | Sulfotransferase | Trehalose | Trehalose-2-sulfate
