1xj9
From Proteopedia
(New page: 200px<br /><applet load="1xj9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xj9, resolution 2.6Å" /> '''Crystal structure of ...) |
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| - | [[Image:1xj9.jpg|left|200px]]<br /><applet load="1xj9" size=" | + | [[Image:1xj9.jpg|left|200px]]<br /><applet load="1xj9" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1xj9, resolution 2.6Å" /> | caption="1xj9, resolution 2.6Å" /> | ||
'''Crystal structure of a partly self-complementary peptide nucleic acid (PNA) oligomer showing a duplex-triplex network'''<br /> | '''Crystal structure of a partly self-complementary peptide nucleic acid (PNA) oligomer showing a duplex-triplex network'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The X-ray structure of a partly self-complementary peptide nucleic acid | + | The X-ray structure of a partly self-complementary peptide nucleic acid (PNA) decamer (H-GTAGATCACT-l-Lys-NH(2)) to 2.60 A resolution is reported. The structure is mainly controlled by the canonical Watson-Crick base pairs formed by the self-complementary stretch of four bases in the middle of the decamer (G(4)A(5)T(6)C(7)). One right- and one left-handed Watson-Crick duplex are formed. The two PNA units C(9)T(10) change helical handedness, so that each PNA strand contains both a right- and a left-handed section. The changed handedness in C(9)T(10) allows formation of Hoogsteen hydrogen bonding between C(9)T(10) and G(4)A(5) of a PNA strand in an adjacent Watson-Crick double helix of the same handedness. Thereby, a PNA-PNA-PNA triplex is formed. The PNA unit A(3) forms a noncanonical base pair with A(8) in a symmetry-related strand of opposite handedness; the base pair is of the A-A reverse Hoogsteen type. The structural diversity of this PNA demonstrates how the PNA backbone is able to adapt to structures governed by the stacking and hydrogen-bonding interactions between the nucleobases. The crystal structure further shows how PNA oligomers containing limited sequence complementarity may form complex hydrogen-bonding networks. |
==About this Structure== | ==About this Structure== | ||
| - | 1XJ9 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1XJ9 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XJ9 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Gajhede, M.]] | [[Category: Gajhede, M.]] | ||
| - | [[Category: Kastrup, J | + | [[Category: Kastrup, J S.]] |
| - | [[Category: Larsen, I | + | [[Category: Larsen, I K.]] |
| - | [[Category: Nielsen, B | + | [[Category: Nielsen, B B.]] |
| - | [[Category: Nielsen, P | + | [[Category: Nielsen, P E.]] |
[[Category: Petersson, B.]] | [[Category: Petersson, B.]] | ||
[[Category: Rasmussen, H.]] | [[Category: Rasmussen, H.]] | ||
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[[Category: right-handed]] | [[Category: right-handed]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:55:33 2008'' |
Revision as of 13:55, 21 February 2008
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Crystal structure of a partly self-complementary peptide nucleic acid (PNA) oligomer showing a duplex-triplex network
Overview
The X-ray structure of a partly self-complementary peptide nucleic acid (PNA) decamer (H-GTAGATCACT-l-Lys-NH(2)) to 2.60 A resolution is reported. The structure is mainly controlled by the canonical Watson-Crick base pairs formed by the self-complementary stretch of four bases in the middle of the decamer (G(4)A(5)T(6)C(7)). One right- and one left-handed Watson-Crick duplex are formed. The two PNA units C(9)T(10) change helical handedness, so that each PNA strand contains both a right- and a left-handed section. The changed handedness in C(9)T(10) allows formation of Hoogsteen hydrogen bonding between C(9)T(10) and G(4)A(5) of a PNA strand in an adjacent Watson-Crick double helix of the same handedness. Thereby, a PNA-PNA-PNA triplex is formed. The PNA unit A(3) forms a noncanonical base pair with A(8) in a symmetry-related strand of opposite handedness; the base pair is of the A-A reverse Hoogsteen type. The structural diversity of this PNA demonstrates how the PNA backbone is able to adapt to structures governed by the stacking and hydrogen-bonding interactions between the nucleobases. The crystal structure further shows how PNA oligomers containing limited sequence complementarity may form complex hydrogen-bonding networks.
About this Structure
1XJ9 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Crystal structure of a partly self-complementary peptide nucleic acid (PNA) oligomer showing a duplex-triplex network., Petersson B, Nielsen BB, Rasmussen H, Larsen IK, Gajhede M, Nielsen PE, Kastrup JS, J Am Chem Soc. 2005 Feb 9;127(5):1424-30. PMID:15686374
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