1l2c
From Proteopedia
(New page: 200px<br /><applet load="1l2c" size="450" color="white" frame="true" align="right" spinBox="true" caption="1l2c, resolution 2.2Å" /> '''MutM (Fpg)-DNA Estran...) |
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| - | [[Image:1l2c.gif|left|200px]]<br /><applet load="1l2c" size=" | + | [[Image:1l2c.gif|left|200px]]<br /><applet load="1l2c" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1l2c, resolution 2.2Å" /> | caption="1l2c, resolution 2.2Å" /> | ||
'''MutM (Fpg)-DNA Estranged Thymine Mismatch Recognition Complex'''<br /> | '''MutM (Fpg)-DNA Estranged Thymine Mismatch Recognition Complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | MutM is a bacterial 8-oxoguanine glycosylase responsible for initiating | + | MutM is a bacterial 8-oxoguanine glycosylase responsible for initiating base-excision repair of oxidized guanine residues in DNA. Here we report five different crystal structures of MutM-DNA complexes that represent different steps of the repair reaction cascade catalyzed by the protein and also differ in the identity of the base opposite the lesion (the 'estranged' base). These structures reveal that the MutM active site performs the multiple steps of base-excision and 3' and 5' nicking with minimal rearrangement of the DNA backbone. |
==About this Structure== | ==About this Structure== | ||
| - | 1L2C is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1L2C is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L2C OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Geobacillus stearothermophilus]] | [[Category: Geobacillus stearothermophilus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Fromme, J | + | [[Category: Fromme, J C.]] |
| - | [[Category: Verdine, G | + | [[Category: Verdine, G L.]] |
[[Category: ZN]] | [[Category: ZN]] | ||
[[Category: dna glycosylase]] | [[Category: dna glycosylase]] | ||
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[[Category: zinc finger]] | [[Category: zinc finger]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:40:28 2008'' |
Revision as of 11:40, 21 February 2008
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MutM (Fpg)-DNA Estranged Thymine Mismatch Recognition Complex
Overview
MutM is a bacterial 8-oxoguanine glycosylase responsible for initiating base-excision repair of oxidized guanine residues in DNA. Here we report five different crystal structures of MutM-DNA complexes that represent different steps of the repair reaction cascade catalyzed by the protein and also differ in the identity of the base opposite the lesion (the 'estranged' base). These structures reveal that the MutM active site performs the multiple steps of base-excision and 3' and 5' nicking with minimal rearrangement of the DNA backbone.
About this Structure
1L2C is a Protein complex structure of sequences from Geobacillus stearothermophilus with as ligand. Full crystallographic information is available from OCA.
Reference
Structural insights into lesion recognition and repair by the bacterial 8-oxoguanine DNA glycosylase MutM., Fromme JC, Verdine GL, Nat Struct Biol. 2002 Jul;9(7):544-52. PMID:12055620
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