User:Wayne Decatur/3fpn Morph methods

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(Morph from normal 3fpn structure to view in Figure 3 of article describing the structure)
(Morph from normal 3fpn structure to view in Figure 3 of article describing the structure)
Line 38: Line 38:
set measurementNumbers OFF
set measurementNumbers OFF
-
then save scene in SCENE AUTHORING TOOS with animation that you play once
+
then SAVE scene in SCENE AUTHORING TOOS with animation that you play once
Line 47: Line 47:
select 170:a and sidechain; wireframe 0.55; color cpk;
select 170:a and sidechain; wireframe 0.55; color cpk;
select 231:b and sidechain; wireframe 0.55;color cpk;
select 231:b and sidechain; wireframe 0.55;color cpk;
 +
 +
then enter in console
 +
model 1.1;
 +
 +
Now go to the view and add measure lines.
Save as palindrome!
Save as palindrome!

Revision as of 05:52, 23 October 2009

Moving to match Figure 3

Using Pymol and the 3fpn file, I moved so interface is perpendicular to y axis:

translate [10,0,0], chain b

rotate y, 65, chain b

Saved molecule.

Morph from normal 3fpn structure to view in Figure 3 of article describing the structure

Insert caption here

Drag the structure with the mouse to rotate

Took the two files and submitted them. Since the structures didn't have nucleic acids, I took the advice here and used the Yale Morph Server for morphing complexes.

Uploaded to Proteopedia Image:3fpntorotatedversion.pdb.
loaded '3fpntorotatedversion.pdb' in Scene Authoring Tools.




Paper on the structure

  • Pakotiprapha D, Liu Y, Verdine GL, Jeruzalmi D. A structural model for the damage-sensing complex in bacterial nucleotide excision repair. J Biol Chem. 2009 May 8;284(19):12837-44. Epub 2009 Mar 13. PMID:19287003 doi:10.1074/jbc.M900571200

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Wayne Decatur

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