1pnn
From Proteopedia
(New page: 200px<br /><applet load="1pnn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pnn, resolution 2.5Å" /> '''PEPTIDE NUCLEIC ACID ...) |
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| - | [[Image:1pnn.gif|left|200px]]<br /><applet load="1pnn" size=" | + | [[Image:1pnn.gif|left|200px]]<br /><applet load="1pnn" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1pnn, resolution 2.5Å" /> | caption="1pnn, resolution 2.5Å" /> | ||
'''PEPTIDE NUCLEIC ACID (PNA) COMPLEXED WITH DNA'''<br /> | '''PEPTIDE NUCLEIC ACID (PNA) COMPLEXED WITH DNA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of a nucleic acid triplex reveals a helix, designated P-form, that differs from previously reported nucleic acid | + | The crystal structure of a nucleic acid triplex reveals a helix, designated P-form, that differs from previously reported nucleic acid structures. The triplex consists of one polypurine DNA strand complexed to a polypyrimidine hairpin peptide nucleic acid (PNA) and was successfully designed to promote Watson-Crick and Hoogsteen base pairing. The P-form helix is underwound, with a base tilt similar to B-form DNA. The bases are displaced from the helix axis even more than in A-form DNA. Hydrogen bonds between the DNA backbone and the Hoogsteen PNA backbone explain the observation that polypyrimidine PNA sequences form highly stable 2:1 PNA-DNA complexes. This structure expands the number of known stable helical forms that nucleic acids can adopt. |
==About this Structure== | ==About this Structure== | ||
| - | 1PNN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1PNN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PNN OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Betts, L.]] | [[Category: Betts, L.]] | ||
| - | [[Category: Veal, J | + | [[Category: Veal, J M.]] |
[[Category: hairpin pna:dna triplex]] | [[Category: hairpin pna:dna triplex]] | ||
[[Category: triplex watson-crick hoogsteen]] | [[Category: triplex watson-crick hoogsteen]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:30:37 2008'' |
Revision as of 12:30, 21 February 2008
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PEPTIDE NUCLEIC ACID (PNA) COMPLEXED WITH DNA
Overview
The crystal structure of a nucleic acid triplex reveals a helix, designated P-form, that differs from previously reported nucleic acid structures. The triplex consists of one polypurine DNA strand complexed to a polypyrimidine hairpin peptide nucleic acid (PNA) and was successfully designed to promote Watson-Crick and Hoogsteen base pairing. The P-form helix is underwound, with a base tilt similar to B-form DNA. The bases are displaced from the helix axis even more than in A-form DNA. Hydrogen bonds between the DNA backbone and the Hoogsteen PNA backbone explain the observation that polypyrimidine PNA sequences form highly stable 2:1 PNA-DNA complexes. This structure expands the number of known stable helical forms that nucleic acids can adopt.
About this Structure
1PNN is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
A nucleic acid triple helix formed by a peptide nucleic acid-DNA complex., Betts L, Josey JA, Veal JM, Jordan SR, Science. 1995 Dec 15;270(5243):1838-41. PMID:8525381
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