1hmh

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(New page: 200px<br /><applet load="1hmh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hmh, resolution 2.600&Aring;" /> '''THREE-DIMENSIONAL S...)
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'''THREE-DIMENSIONAL STRUCTURE OF A HAMMERHEAD RIBOZYME'''<br />
'''THREE-DIMENSIONAL STRUCTURE OF A HAMMERHEAD RIBOZYME'''<br />
==Overview==
==Overview==
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The hammerhead ribozyme is a small catalytic RNA motif made up of three, base-paired stems and a core of highly conserved, non-complementary, nucleotides essential for catalysis. The X-ray crystallographic structure, of a hammerhead RNA-DNA ribozyme-inhibitor complex at 2.6 A resolution, reveals that the base-paired stems are A-form helices and that the core, has two structural domains. The first domain is formed by the sequence, 5'-CUGA following stem I and is a sharp turn identical to the uridine turn, of transfer RNA, whereas the second is a non-Watson-Crick three-base-pair, duplex with a divalent-ion binding site. The phosphodiester backbone of, the DNA inhibitor strand is splayed out at the phosphate 5' to the, cleavage site. The structure indicates that the ribozyme may destabilize a, substrate strand in order to facilitate twisting of the substrate to allow, cleavage of the scissile bond.
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The hammerhead ribozyme is a small catalytic RNA motif made up of three base-paired stems and a core of highly conserved, non-complementary nucleotides essential for catalysis. The X-ray crystallographic structure of a hammerhead RNA-DNA ribozyme-inhibitor complex at 2.6 A resolution reveals that the base-paired stems are A-form helices and that the core has two structural domains. The first domain is formed by the sequence 5'-CUGA following stem I and is a sharp turn identical to the uridine turn of transfer RNA, whereas the second is a non-Watson-Crick three-base-pair duplex with a divalent-ion binding site. The phosphodiester backbone of the DNA inhibitor strand is splayed out at the phosphate 5' to the cleavage site. The structure indicates that the ribozyme may destabilize a substrate strand in order to facilitate twisting of the substrate to allow cleavage of the scissile bond.
==About this Structure==
==About this Structure==
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1HMH is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HMH OCA].
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1HMH is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HMH OCA].
==Reference==
==Reference==
Three-dimensional structure of a hammerhead ribozyme., Pley HW, Flaherty KM, McKay DB, Nature. 1994 Nov 3;372(6501):68-74. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7969422 7969422]
Three-dimensional structure of a hammerhead ribozyme., Pley HW, Flaherty KM, McKay DB, Nature. 1994 Nov 3;372(6501):68-74. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7969422 7969422]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: Flaherty, K.M.]]
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[[Category: Flaherty, K M.]]
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[[Category: McKay, D.B.]]
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[[Category: McKay, D B.]]
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[[Category: Pley, H.W.]]
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[[Category: Pley, H W.]]
[[Category: dna-rna hammerhead ribozyme]]
[[Category: dna-rna hammerhead ribozyme]]
[[Category: loop]]
[[Category: loop]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:47:06 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:02:47 2008''

Revision as of 11:02, 21 February 2008

Image:1hmh.jpg

1hmh, resolution 2.600Å

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THREE-DIMENSIONAL STRUCTURE OF A HAMMERHEAD RIBOZYME

Overview

The hammerhead ribozyme is a small catalytic RNA motif made up of three base-paired stems and a core of highly conserved, non-complementary nucleotides essential for catalysis. The X-ray crystallographic structure of a hammerhead RNA-DNA ribozyme-inhibitor complex at 2.6 A resolution reveals that the base-paired stems are A-form helices and that the core has two structural domains. The first domain is formed by the sequence 5'-CUGA following stem I and is a sharp turn identical to the uridine turn of transfer RNA, whereas the second is a non-Watson-Crick three-base-pair duplex with a divalent-ion binding site. The phosphodiester backbone of the DNA inhibitor strand is splayed out at the phosphate 5' to the cleavage site. The structure indicates that the ribozyme may destabilize a substrate strand in order to facilitate twisting of the substrate to allow cleavage of the scissile bond.

About this Structure

1HMH is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

Three-dimensional structure of a hammerhead ribozyme., Pley HW, Flaherty KM, McKay DB, Nature. 1994 Nov 3;372(6501):68-74. PMID:7969422

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