1lc4
From Proteopedia
(New page: 200px<br /><applet load="1lc4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lc4, resolution 2.54Å" /> '''Crystal Structure of...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1lc4.gif|left|200px]]<br /><applet load="1lc4" size=" | + | [[Image:1lc4.gif|left|200px]]<br /><applet load="1lc4" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1lc4, resolution 2.54Å" /> | caption="1lc4, resolution 2.54Å" /> | ||
'''Crystal Structure of Tobramycin Bound to the Eubacterial 16S rRNA A Site'''<br /> | '''Crystal Structure of Tobramycin Bound to the Eubacterial 16S rRNA A Site'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Aminoglycoside antibiotics target the decoding aminoacyl site (A site) on | + | Aminoglycoside antibiotics target the decoding aminoacyl site (A site) on the 16S ribosomal RNA and induce miscoding during translation. Here, we present the crystal structure, at 2.54 A resolution, of an RNA oligonucleotide containing the A site sequence complexed to the 4,6-disubstituted 2-deoxystreptamine aminoglycoside tobramycin. The three aminosugar rings making up tobramycin interact with the deep-groove atoms directly or via water molecules and stabilize a fully bulged-out conformation of adenines A(1492) and A(1493). The comparison between this structure and the one previously solved in the presence of paromomycin confirms the importance of the functional groups on the common neamine part of these two antibiotics for binding to RNA. Furthermore, the analysis of the present structure provides a molecular explanation to some of the resistance mechanisms that have spread among bacteria and rendered aminoglycoside antibiotics inefficient. |
==About this Structure== | ==About this Structure== | ||
| - | 1LC4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with TOY as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1LC4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=TOY:'>TOY</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LC4 OCA]. |
==Reference== | ==Reference== | ||
| Line 18: | Line 18: | ||
[[Category: antibiotic-rna complex]] | [[Category: antibiotic-rna complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:43:34 2008'' |
Revision as of 11:43, 21 February 2008
|
Crystal Structure of Tobramycin Bound to the Eubacterial 16S rRNA A Site
Overview
Aminoglycoside antibiotics target the decoding aminoacyl site (A site) on the 16S ribosomal RNA and induce miscoding during translation. Here, we present the crystal structure, at 2.54 A resolution, of an RNA oligonucleotide containing the A site sequence complexed to the 4,6-disubstituted 2-deoxystreptamine aminoglycoside tobramycin. The three aminosugar rings making up tobramycin interact with the deep-groove atoms directly or via water molecules and stabilize a fully bulged-out conformation of adenines A(1492) and A(1493). The comparison between this structure and the one previously solved in the presence of paromomycin confirms the importance of the functional groups on the common neamine part of these two antibiotics for binding to RNA. Furthermore, the analysis of the present structure provides a molecular explanation to some of the resistance mechanisms that have spread among bacteria and rendered aminoglycoside antibiotics inefficient.
About this Structure
1LC4 is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of a complex between the aminoglycoside tobramycin and an oligonucleotide containing the ribosomal decoding a site., Vicens Q, Westhof E, Chem Biol. 2002 Jun;9(6):747-55. PMID:12079787
Page seeded by OCA on Thu Feb 21 13:43:34 2008
