1lcm
From Proteopedia
(New page: 200px<br /><applet load="1lcm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lcm" /> '''MICROCYSTIN-LR, NMR, MINIMIZED AVERAGE STRUC...) |
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| - | [[Image:1lcm.jpg|left|200px]]<br /><applet load="1lcm" size=" | + | [[Image:1lcm.jpg|left|200px]]<br /><applet load="1lcm" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''MICROCYSTIN-LR, NMR, MINIMIZED AVERAGE STRUCTURE'''<br /> | '''MICROCYSTIN-LR, NMR, MINIMIZED AVERAGE STRUCTURE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | NMR spectroscopy in aqueous and dimethyl sulfoxide/water solutions is used | + | NMR spectroscopy in aqueous and dimethyl sulfoxide/water solutions is used to determine the three-dimensional structures of microcystin-LR, a cyclic cyanobacterial heptapeptide toxin which is a potent inhibitor of type 1 and type 2A protein phosphatases. The conformations of this toxic peptide are studied using a simulated annealing (SA) protocol followed by refined SA calculations in vacuo and free MD simulations in water. Only one conformational family in each solvent is found. The peptide ring has a saddle-shaped form, essentially the same in both solvents. The structural difference observed between the two solution structures is located to the part consisting of Mdha, Ala, and Leu. This peptide segment is not present in nodularin, a cyclic pentapeptide of similar toxicity. The Arg side chain is very flexible, while the side chain of Leu is well defined. The side chain of Adda, essential for toxicity, is constrained in the vicinity of the backbone ring but appears to be flexible in the more remote part. |
==About this Structure== | ==About this Structure== | ||
| - | 1LCM is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Microcystis_aeruginosa Microcystis aeruginosa]. Full crystallographic information is available from [http:// | + | 1LCM is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Microcystis_aeruginosa Microcystis aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LCM OCA]. |
==Reference== | ==Reference== | ||
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[[Category: cyanobacterial toxin]] | [[Category: cyanobacterial toxin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:43:44 2008'' |
Revision as of 11:43, 21 February 2008
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MICROCYSTIN-LR, NMR, MINIMIZED AVERAGE STRUCTURE
Overview
NMR spectroscopy in aqueous and dimethyl sulfoxide/water solutions is used to determine the three-dimensional structures of microcystin-LR, a cyclic cyanobacterial heptapeptide toxin which is a potent inhibitor of type 1 and type 2A protein phosphatases. The conformations of this toxic peptide are studied using a simulated annealing (SA) protocol followed by refined SA calculations in vacuo and free MD simulations in water. Only one conformational family in each solvent is found. The peptide ring has a saddle-shaped form, essentially the same in both solvents. The structural difference observed between the two solution structures is located to the part consisting of Mdha, Ala, and Leu. This peptide segment is not present in nodularin, a cyclic pentapeptide of similar toxicity. The Arg side chain is very flexible, while the side chain of Leu is well defined. The side chain of Adda, essential for toxicity, is constrained in the vicinity of the backbone ring but appears to be flexible in the more remote part.
About this Structure
1LCM is a Protein complex structure of sequences from Microcystis aeruginosa. Full crystallographic information is available from OCA.
Reference
Conformational studies of microcystin-LR using NMR spectroscopy and molecular dynamics calculations., Trogen GB, Annila A, Eriksson J, Kontteli M, Meriluoto J, Sethson I, Zdunek J, Edlund U, Biochemistry. 1996 Mar 12;35(10):3197-205. PMID:8605154
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