1lf6
From Proteopedia
(New page: 200px<br /><applet load="1lf6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lf6, resolution 2.10Å" /> '''CRYSTAL STRUCTURE OF...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1lf6.gif|left|200px]]<br /><applet load="1lf6" size=" | + | [[Image:1lf6.gif|left|200px]]<br /><applet load="1lf6" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1lf6, resolution 2.10Å" /> | caption="1lf6, resolution 2.10Å" /> | ||
'''CRYSTAL STRUCTURE OF BACTERIAL GLUCOAMYLASE'''<br /> | '''CRYSTAL STRUCTURE OF BACTERIAL GLUCOAMYLASE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The first crystal structures of a two-domain, prokaryotic glucoamylase | + | The first crystal structures of a two-domain, prokaryotic glucoamylase were determined to high resolution from the clostridial species Thermoanaerobacterium thermosaccharolyticum with and without acarbose. The N-terminal domain has 18 antiparallel strands arranged in beta-sheets of a super-beta-sandwich. The C-terminal domain is an (alpha/alpha)(6) barrel, lacking the peripheral subdomain of eukaryotic glucoamylases. Interdomain contacts are common to all prokaryotic Family GH15 proteins. Domains similar to those of prokaryotic glucoamylases in maltose phosphorylases (Family GH65) and glycoaminoglycan lyases (Family PL8) suggest evolution from a common ancestor. Eukaryotic glucoamylases may have evolved from prokaryotic glucoamylases by the substitution of the N-terminal domain with the peripheral subdomain and by the addition of a starch-binding domain. |
==About this Structure== | ==About this Structure== | ||
| - | 1LF6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermoanaerobacterium_thermosaccharolyticum Thermoanaerobacterium thermosaccharolyticum] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Glucan_1,4-alpha-glucosidase Glucan 1,4-alpha-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.3 3.2.1.3] Full crystallographic information is available from [http:// | + | 1LF6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermoanaerobacterium_thermosaccharolyticum Thermoanaerobacterium thermosaccharolyticum] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Glucan_1,4-alpha-glucosidase Glucan 1,4-alpha-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.3 3.2.1.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LF6 OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thermoanaerobacterium thermosaccharolyticum]] | [[Category: Thermoanaerobacterium thermosaccharolyticum]] | ||
| - | [[Category: Aleshin, A | + | [[Category: Aleshin, A E.]] |
| - | [[Category: Feng, P | + | [[Category: Feng, P H.]] |
| - | [[Category: Honzatko, R | + | [[Category: Honzatko, R B.]] |
| - | [[Category: Reilly, P | + | [[Category: Reilly, P J.]] |
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: (alpha/alpha) barrel]] | [[Category: (alpha/alpha) barrel]] | ||
| Line 24: | Line 24: | ||
[[Category: super beta sandwich]] | [[Category: super beta sandwich]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:44:26 2008'' |
Revision as of 11:44, 21 February 2008
|
CRYSTAL STRUCTURE OF BACTERIAL GLUCOAMYLASE
Overview
The first crystal structures of a two-domain, prokaryotic glucoamylase were determined to high resolution from the clostridial species Thermoanaerobacterium thermosaccharolyticum with and without acarbose. The N-terminal domain has 18 antiparallel strands arranged in beta-sheets of a super-beta-sandwich. The C-terminal domain is an (alpha/alpha)(6) barrel, lacking the peripheral subdomain of eukaryotic glucoamylases. Interdomain contacts are common to all prokaryotic Family GH15 proteins. Domains similar to those of prokaryotic glucoamylases in maltose phosphorylases (Family GH65) and glycoaminoglycan lyases (Family PL8) suggest evolution from a common ancestor. Eukaryotic glucoamylases may have evolved from prokaryotic glucoamylases by the substitution of the N-terminal domain with the peripheral subdomain and by the addition of a starch-binding domain.
About this Structure
1LF6 is a Single protein structure of sequence from Thermoanaerobacterium thermosaccharolyticum with as ligand. Active as Glucan 1,4-alpha-glucosidase, with EC number 3.2.1.3 Full crystallographic information is available from OCA.
Reference
Crystal structure and evolution of a prokaryotic glucoamylase., Aleshin AE, Feng PH, Honzatko RB, Reilly PJ, J Mol Biol. 2003 Mar 14;327(1):61-73. PMID:12614608
Page seeded by OCA on Thu Feb 21 13:44:26 2008
