This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1ddp
From Proteopedia
(New page: 200px<br /><applet load="1ddp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ddp" /> '''SOLUTION STRUCTURE OF A CISPLATIN-INDUCED [C...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1ddp.gif|left|200px]]<br /><applet load="1ddp" size=" | + | [[Image:1ddp.gif|left|200px]]<br /><applet load="1ddp" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ddp" /> | caption="1ddp" /> | ||
'''SOLUTION STRUCTURE OF A CISPLATIN-INDUCED [CATAGCTATG]2 INTERSTRAND CROSS-LINK'''<br /> | '''SOLUTION STRUCTURE OF A CISPLATIN-INDUCED [CATAGCTATG]2 INTERSTRAND CROSS-LINK'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The widely used antitumor drug cis-diamminedichloroplatinum(II) (cisplatin | + | The widely used antitumor drug cis-diamminedichloroplatinum(II) (cisplatin or cis-DDP) reacts with DNA, cross-linking two purine residues through the N7 atoms, which reside in the major groove in B-form DNA. The solution structure of the short duplex [d(CAT-AGCTATG)]2 cross-linked at the GC:GC site was determined by nuclear magnetic resonance (NMR). The deoxyguanosine-bridging cis-diammineplatinum(II) lies in the minor groove, and the complementary deoxycytidines are extrahelical. The double helix is locally reversed to a left-handed form, and the helix is unwound and bent toward the minor groove. These findings were independently confirmed by results from a phase-sensitive gel electrophoresis bending assay. The NMR structure differs markedly from previously proposed models but accounts for the chemical reactivity, the unwinding, and the bending of cis-DDP interstrand cross-linked DNA and may be important in the formation and repair of these cross-links in chromatin. |
==About this Structure== | ==About this Structure== | ||
| - | 1DDP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with CPT as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1DDP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CPT:'>CPT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DDP OCA]. |
==Reference== | ==Reference== | ||
Solution structure of a cisplatin-induced DNA interstrand cross-link., Huang H, Zhu L, Reid BR, Drobny GP, Hopkins PB, Science. 1995 Dec 15;270(5243):1842-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8525382 8525382] | Solution structure of a cisplatin-induced DNA interstrand cross-link., Huang H, Zhu L, Reid BR, Drobny GP, Hopkins PB, Science. 1995 Dec 15;270(5243):1842-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8525382 8525382] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Drobny, G | + | [[Category: Drobny, G P.]] |
| - | [[Category: Hopkins, P | + | [[Category: Hopkins, P B.]] |
[[Category: Huang, H.]] | [[Category: Huang, H.]] | ||
| - | [[Category: Reid, B | + | [[Category: Reid, B R.]] |
[[Category: Zhu, L.]] | [[Category: Zhu, L.]] | ||
[[Category: CPT]] | [[Category: CPT]] | ||
| Line 25: | Line 25: | ||
[[Category: nmr]] | [[Category: nmr]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:15:38 2008'' |
Revision as of 10:15, 21 February 2008
|
SOLUTION STRUCTURE OF A CISPLATIN-INDUCED [CATAGCTATG]2 INTERSTRAND CROSS-LINK
Overview
The widely used antitumor drug cis-diamminedichloroplatinum(II) (cisplatin or cis-DDP) reacts with DNA, cross-linking two purine residues through the N7 atoms, which reside in the major groove in B-form DNA. The solution structure of the short duplex [d(CAT-AGCTATG)]2 cross-linked at the GC:GC site was determined by nuclear magnetic resonance (NMR). The deoxyguanosine-bridging cis-diammineplatinum(II) lies in the minor groove, and the complementary deoxycytidines are extrahelical. The double helix is locally reversed to a left-handed form, and the helix is unwound and bent toward the minor groove. These findings were independently confirmed by results from a phase-sensitive gel electrophoresis bending assay. The NMR structure differs markedly from previously proposed models but accounts for the chemical reactivity, the unwinding, and the bending of cis-DDP interstrand cross-linked DNA and may be important in the formation and repair of these cross-links in chromatin.
About this Structure
1DDP is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Solution structure of a cisplatin-induced DNA interstrand cross-link., Huang H, Zhu L, Reid BR, Drobny GP, Hopkins PB, Science. 1995 Dec 15;270(5243):1842-5. PMID:8525382
Page seeded by OCA on Thu Feb 21 12:15:38 2008
Categories: Protein complex | Drobny, G P. | Hopkins, P B. | Huang, H. | Reid, B R. | Zhu, L. | CPT | Antitumor drug | Cis-ddp | Dna | Double helix | Interstrand cross-link | Nmr
