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1xvx
From Proteopedia
(New page: 200px<br /><applet load="1xvx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xvx, resolution 1.53Å" /> '''Crystal Structure of...) |
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| - | [[Image:1xvx.gif|left|200px]]<br /><applet load="1xvx" size=" | + | [[Image:1xvx.gif|left|200px]]<br /><applet load="1xvx" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1xvx, resolution 1.53Å" /> | caption="1xvx, resolution 1.53Å" /> | ||
'''Crystal Structure of iron-loaded Yersinia enterocolitica YfuA'''<br /> | '''Crystal Structure of iron-loaded Yersinia enterocolitica YfuA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The uptake of the element iron is vital for the survival of most | + | The uptake of the element iron is vital for the survival of most organisms. Numerous pathogenic Gram-negative bacteria utilize a periplasm-to-cytosol ATP-binding cassette transport pathway to transport this essential atom in to the cell. In this study, we investigated the Yersinia enterocolitica (YfuA) and Serratia marcescens (SfuA) iron-binding periplasmic proteins. We have determined the 1.8-angstroms structures of iron-loaded (YfuA) and iron-free (SfuA) forms of this class of proteins. Although the sequence of these proteins varies considerably from the other members of the transferrin structural superfamily, they adopt the same three-dimensional fold. The iron-loaded YfuA structure illustrates the unique nature of this new class of proteins in that they are able to octahedrally coordinate the ferric ion in the absence of a bound anion. The iron-free SfuA structure contains a bound citrate anion in the iron-binding cleft that tethers the N- and C-terminal domains of the apo protein and stabilizes the partially open structure. |
==About this Structure== | ==About this Structure== | ||
| - | 1XVX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Yersinia_enterocolitica Yersinia enterocolitica] with FE, ZN, CO3 and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1XVX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Yersinia_enterocolitica Yersinia enterocolitica] with <scene name='pdbligand=FE:'>FE</scene>, <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=CO3:'>CO3</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XVX OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Yersinia enterocolitica]] | [[Category: Yersinia enterocolitica]] | ||
| - | [[Category: Dougan, D | + | [[Category: Dougan, D R.]] |
| - | [[Category: McRee, D | + | [[Category: McRee, D E.]] |
| - | [[Category: Schryvers, A | + | [[Category: Schryvers, A B.]] |
| - | [[Category: Shouldice, S | + | [[Category: Shouldice, S R.]] |
| - | [[Category: Tari, L | + | [[Category: Tari, L W.]] |
[[Category: CO3]] | [[Category: CO3]] | ||
[[Category: EDO]] | [[Category: EDO]] | ||
| Line 24: | Line 24: | ||
[[Category: periplasmic iron binding protein]] | [[Category: periplasmic iron binding protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:59:14 2008'' |
Revision as of 13:59, 21 February 2008
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Crystal Structure of iron-loaded Yersinia enterocolitica YfuA
Overview
The uptake of the element iron is vital for the survival of most organisms. Numerous pathogenic Gram-negative bacteria utilize a periplasm-to-cytosol ATP-binding cassette transport pathway to transport this essential atom in to the cell. In this study, we investigated the Yersinia enterocolitica (YfuA) and Serratia marcescens (SfuA) iron-binding periplasmic proteins. We have determined the 1.8-angstroms structures of iron-loaded (YfuA) and iron-free (SfuA) forms of this class of proteins. Although the sequence of these proteins varies considerably from the other members of the transferrin structural superfamily, they adopt the same three-dimensional fold. The iron-loaded YfuA structure illustrates the unique nature of this new class of proteins in that they are able to octahedrally coordinate the ferric ion in the absence of a bound anion. The iron-free SfuA structure contains a bound citrate anion in the iron-binding cleft that tethers the N- and C-terminal domains of the apo protein and stabilizes the partially open structure.
About this Structure
1XVX is a Single protein structure of sequence from Yersinia enterocolitica with , , and as ligands. Full crystallographic information is available from OCA.
Reference
Novel anion-independent iron coordination by members of a third class of bacterial periplasmic ferric ion-binding proteins., Shouldice SR, McRee DE, Dougan DR, Tari LW, Schryvers AB, J Biol Chem. 2005 Feb 18;280(7):5820-7. Epub 2004 Dec 2. PMID:15576371
Page seeded by OCA on Thu Feb 21 15:59:14 2008
