1u11
From Proteopedia
(New page: 200px<br /><applet load="1u11" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u11, resolution 1.55Å" /> '''PurE (N5-carboxyamin...) |
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| - | [[Image:1u11.gif|left|200px]]<br /><applet load="1u11" size=" | + | [[Image:1u11.gif|left|200px]]<br /><applet load="1u11" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1u11, resolution 1.55Å" /> | caption="1u11, resolution 1.55Å" /> | ||
'''PurE (N5-carboxyaminoimidazole Ribonucleotide Mutase) from the acidophile Acetobacter aceti'''<br /> | '''PurE (N5-carboxyaminoimidazole Ribonucleotide Mutase) from the acidophile Acetobacter aceti'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of Acetobacter aceti PurE was determined to a | + | The crystal structure of Acetobacter aceti PurE was determined to a resolution of 1.55 A and is compared with the known structures of the class I PurEs from a mesophile, Escherichia coli, and a thermophile, Thermotoga maritima. Analyses of the general factors that increase protein stability are examined as potential explanations for the acid stability of A. aceti PurE. Increased inter-subunit hydrogen bonding and an increased number of arginine-containing salt bridges appear to account for the bulk of the increased acid stability. A chain of histidines linking two active sites is discussed in the context of the proton transfers catalyzed by the enzyme. |
==About this Structure== | ==About this Structure== | ||
| - | 1U11 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Acetobacter_aceti Acetobacter aceti] with CIT as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1U11 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Acetobacter_aceti Acetobacter aceti] with <scene name='pdbligand=CIT:'>CIT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U11 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Acetobacter aceti]] | [[Category: Acetobacter aceti]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Chittuluru, J | + | [[Category: Chittuluru, J R.]] |
| - | [[Category: Ealick, S | + | [[Category: Ealick, S E.]] |
| - | [[Category: Kappock, T | + | [[Category: Kappock, T J.]] |
| - | [[Category: Mill, C | + | [[Category: Mill, C P.]] |
| - | [[Category: Settembre, E | + | [[Category: Settembre, E C.]] |
[[Category: CIT]] | [[Category: CIT]] | ||
[[Category: acidophile]] | [[Category: acidophile]] | ||
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[[Category: pure]] | [[Category: pure]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:19:29 2008'' |
Revision as of 13:19, 21 February 2008
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PurE (N5-carboxyaminoimidazole Ribonucleotide Mutase) from the acidophile Acetobacter aceti
Overview
The crystal structure of Acetobacter aceti PurE was determined to a resolution of 1.55 A and is compared with the known structures of the class I PurEs from a mesophile, Escherichia coli, and a thermophile, Thermotoga maritima. Analyses of the general factors that increase protein stability are examined as potential explanations for the acid stability of A. aceti PurE. Increased inter-subunit hydrogen bonding and an increased number of arginine-containing salt bridges appear to account for the bulk of the increased acid stability. A chain of histidines linking two active sites is discussed in the context of the proton transfers catalyzed by the enzyme.
About this Structure
1U11 is a Protein complex structure of sequences from Acetobacter aceti with as ligand. Full crystallographic information is available from OCA.
Reference
Acidophilic adaptations in the structure of Acetobacter aceti N5-carboxyaminoimidazole ribonucleotide mutase (PurE)., Settembre EC, Chittuluru JR, Mill CP, Kappock TJ, Ealick SE, Acta Crystallogr D Biol Crystallogr. 2004 Oct;60(Pt 10):1753-60. Epub 2004, Sep 23. PMID:15388921
Page seeded by OCA on Thu Feb 21 15:19:29 2008
