1lmv
From Proteopedia
(New page: 200px<br /><applet load="1lmv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lmv" /> '''Solution structure of the unmodified U2 snRN...) |
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'''Solution structure of the unmodified U2 snRNA-intron branch site helix from S. cerevisiae'''<br /> | '''Solution structure of the unmodified U2 snRNA-intron branch site helix from S. cerevisiae'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Pairing of a consensus sequence of the precursor (pre)-mRNA intron with a | + | Pairing of a consensus sequence of the precursor (pre)-mRNA intron with a short region of the U2 small nuclear (sn)RNA during assembly of the eukaryotic spliceosome results in formation of a complementary helix of seven base pairs with a single unpaired adenosine residue. The 2' OH of this adenosine, called the branch site, brings about nucleophilic attack at the pre-mRNA 5' splice site in the first step of splicing. Another feature of this pairing is the phylogenetic conservation of a pseudouridine (psi) residue in U2 snRNA nearly opposite the branch site. We show that the presence of this psi in the pre-mRNA branch-site helix of Saccharomyces cerevisiae induces a dramatically altered architectural landscape compared with that of its unmodified counterpart. The psi-induced structure places the nucleophile in an accessible position for the first step of splicing. |
==About this Structure== | ==About this Structure== | ||
| - | 1LMV is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http:// | + | 1LMV is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LMV OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
| - | [[Category: Greenbaum, N | + | [[Category: Greenbaum, N L.]] |
| - | [[Category: Newby, M | + | [[Category: Newby, M I.]] |
[[Category: a-form helix]] | [[Category: a-form helix]] | ||
[[Category: branch site]] | [[Category: branch site]] | ||
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[[Category: u2 snrna]] | [[Category: u2 snrna]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:46:31 2008'' |
Revision as of 11:46, 21 February 2008
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Solution structure of the unmodified U2 snRNA-intron branch site helix from S. cerevisiae
Overview
Pairing of a consensus sequence of the precursor (pre)-mRNA intron with a short region of the U2 small nuclear (sn)RNA during assembly of the eukaryotic spliceosome results in formation of a complementary helix of seven base pairs with a single unpaired adenosine residue. The 2' OH of this adenosine, called the branch site, brings about nucleophilic attack at the pre-mRNA 5' splice site in the first step of splicing. Another feature of this pairing is the phylogenetic conservation of a pseudouridine (psi) residue in U2 snRNA nearly opposite the branch site. We show that the presence of this psi in the pre-mRNA branch-site helix of Saccharomyces cerevisiae induces a dramatically altered architectural landscape compared with that of its unmodified counterpart. The psi-induced structure places the nucleophile in an accessible position for the first step of splicing.
About this Structure
1LMV is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Sculpting of the spliceosomal branch site recognition motif by a conserved pseudouridine., Newby MI, Greenbaum NL, Nat Struct Biol. 2002 Dec;9(12):958-65. PMID:12426583
Page seeded by OCA on Thu Feb 21 13:46:31 2008
