1hkc

From Proteopedia

Revision as of 08:41, 30 October 2007

RECOMBINANT HUMAN HEXOKINASE TYPE I COMPLEXED WITH GLUCOSE AND PHOSPHATE

Overview

Hexokinase I, the pacemaker of glycolysis in brain tissue and red blood, cells, is comprised of two similar domains fused into a single polypeptide, chain. The C-terminal half of hexokinase I is catalytically active, whereas the N-terminal half is necessary for the relief of product, inhibition by phosphate. A crystalline complex of recombinant human, hexokinase I with glucose and phosphate (2.8 A resolution) reveals a, single binding site for phosphate and glucose at the N-terminal half of, the enzyme. Glucose and phosphate stabilize the N-terminal half in a, closed conformation. Unexpectedly, glucose binds weakly to the C-terminal, half of the enzyme and does not by itself stabilize a closed conformation., Evidently a stable, closed C-terminal half requires either ATP or glucose, ... [(full description)]

About this Structure

1HKC is a [Single protein] structure of sequence from [Homo sapiens] with GLC, PO4 and K as [ligands]. Active as [Hexokinase], with EC number [2.7.1.1]. Structure known Active Sites: GLN, MEC, MEN, PIC and PIN. Full crystallographic information is available from [OCA].

Reference

Regulation of hexokinase I: crystal structure of recombinant human brain hexokinase complexed with glucose and phosphate., Aleshin AE, Zeng C, Bartunik HD, Fromm HJ, Honzatko RB, J Mol Biol. 1998 Sep 18;282(2):345-57. PMID:9735292

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