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1u8d
From Proteopedia
(New page: 200px<br /><applet load="1u8d" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u8d, resolution 1.95Å" /> '''Guanine riboswitch b...) |
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| - | [[Image:1u8d.gif|left|200px]]<br /><applet load="1u8d" size=" | + | [[Image:1u8d.gif|left|200px]]<br /><applet load="1u8d" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1u8d, resolution 1.95Å" /> | caption="1u8d, resolution 1.95Å" /> | ||
'''Guanine riboswitch bound to hypoxanthine'''<br /> | '''Guanine riboswitch bound to hypoxanthine'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Riboswitches are genetic regulatory elements found in the 5' untranslated | + | Riboswitches are genetic regulatory elements found in the 5' untranslated region of messenger RNA that act in the absence of protein cofactors. They are broadly distributed across bacteria and account for the regulation of more than 2% of all genes in Bacillus subtilis, underscoring their importance in the control of cellular metabolism. The 5' untranslated region of many mRNAs of genes involved in purine metabolism and transport contain a guanine-responsive riboswitch that directly binds guanine, hypoxanthine or xanthine to terminate transcription. Here we report the crystal structure at 1.95 A resolution of the purine-binding domain of the guanine riboswitch from the xpt-pbuX operon of B. subtilis bound to hypoxanthine, a prevalent metabolite in the bacterial purine salvage pathway. This structure reveals a complex RNA fold involving several phylogenetically conserved nucleotides that create a binding pocket that almost completely envelops the ligand. Hypoxanthine functions to stabilize this structure and to promote the formation of a downstream transcriptional terminator element, thereby providing a mechanism for directly repressing gene expression in response to an increase in intracellular concentrations of metabolite. |
==About this Structure== | ==About this Structure== | ||
| - | 1U8D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with SPD, ACT, NCO and HPA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1U8D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=SPD:'>SPD</scene>, <scene name='pdbligand=ACT:'>ACT</scene>, <scene name='pdbligand=NCO:'>NCO</scene> and <scene name='pdbligand=HPA:'>HPA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U8D OCA]. |
==Reference== | ==Reference== | ||
Structure of a natural guanine-responsive riboswitch complexed with the metabolite hypoxanthine., Batey RT, Gilbert SD, Montange RK, Nature. 2004 Nov 18;432(7015):411-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15549109 15549109] | Structure of a natural guanine-responsive riboswitch complexed with the metabolite hypoxanthine., Batey RT, Gilbert SD, Montange RK, Nature. 2004 Nov 18;432(7015):411-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15549109 15549109] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Batey, R | + | [[Category: Batey, R T.]] |
| - | [[Category: Gilbert, S | + | [[Category: Gilbert, S D.]] |
| - | [[Category: Montange, R | + | [[Category: Montange, R K.]] |
[[Category: ACT]] | [[Category: ACT]] | ||
[[Category: HPA]] | [[Category: HPA]] | ||
| Line 25: | Line 25: | ||
[[Category: rna-ligand complex]] | [[Category: rna-ligand complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:21:40 2008'' |
Revision as of 13:21, 21 February 2008
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Guanine riboswitch bound to hypoxanthine
Overview
Riboswitches are genetic regulatory elements found in the 5' untranslated region of messenger RNA that act in the absence of protein cofactors. They are broadly distributed across bacteria and account for the regulation of more than 2% of all genes in Bacillus subtilis, underscoring their importance in the control of cellular metabolism. The 5' untranslated region of many mRNAs of genes involved in purine metabolism and transport contain a guanine-responsive riboswitch that directly binds guanine, hypoxanthine or xanthine to terminate transcription. Here we report the crystal structure at 1.95 A resolution of the purine-binding domain of the guanine riboswitch from the xpt-pbuX operon of B. subtilis bound to hypoxanthine, a prevalent metabolite in the bacterial purine salvage pathway. This structure reveals a complex RNA fold involving several phylogenetically conserved nucleotides that create a binding pocket that almost completely envelops the ligand. Hypoxanthine functions to stabilize this structure and to promote the formation of a downstream transcriptional terminator element, thereby providing a mechanism for directly repressing gene expression in response to an increase in intracellular concentrations of metabolite.
About this Structure
1U8D is a Protein complex structure of sequences from [1] with , , and as ligands. Full crystallographic information is available from OCA.
Reference
Structure of a natural guanine-responsive riboswitch complexed with the metabolite hypoxanthine., Batey RT, Gilbert SD, Montange RK, Nature. 2004 Nov 18;432(7015):411-5. PMID:15549109
Page seeded by OCA on Thu Feb 21 15:21:40 2008
Categories: Protein complex | Batey, R T. | Gilbert, S D. | Montange, R K. | ACT | HPA | NCO | SPD | Base quadruples | Base triples | Double helix | Mrna | Rna-ligand complex
