1i47

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(New page: 200px<br /><applet load="1i47" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i47, resolution 2.1&Aring;" /> '''MOLECULAR AND CRYSTAL...)
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'''MOLECULAR AND CRYSTAL STRUCTURE OF D(CGCGAATT(MO4)CGCG): THE WATSON-CRICK TYPE AND WOBBLE N4-METHOXYCYTIDINE/GUANOSINE BASE PAIRS IN B-DNA'''<br />
'''MOLECULAR AND CRYSTAL STRUCTURE OF D(CGCGAATT(MO4)CGCG): THE WATSON-CRICK TYPE AND WOBBLE N4-METHOXYCYTIDINE/GUANOSINE BASE PAIRS IN B-DNA'''<br />
==Overview==
==Overview==
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To investigate the mutation mechanism of purine transition in DNA damaged, with methoxyamine, a DNA dodecamer with the sequence d(CGCGAATTmo(4)CGCG), where mo(4)C is 2'-deoxy-N(4)-methoxycytidine, has been synthesized and, its crystal structure determined. Two dodecamers form a B-form duplex., Electron density maps clearly show that one of the two mo(4)C residues, forms a pair with a guanine residue of the opposite strand, the geometry, being the canonical Watson-Crick type, and that the other mo(4)C residue, forms a wobble pair with the opposite guanine residue. These two pairings, are ascribed to the tautomerization of the methoxylated cytosine moieties, between the amino and imino forms.
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To investigate the mutation mechanism of purine transition in DNA damaged with methoxyamine, a DNA dodecamer with the sequence d(CGCGAATTmo(4)CGCG), where mo(4)C is 2'-deoxy-N(4)-methoxycytidine, has been synthesized and its crystal structure determined. Two dodecamers form a B-form duplex. Electron density maps clearly show that one of the two mo(4)C residues forms a pair with a guanine residue of the opposite strand, the geometry being the canonical Watson-Crick type, and that the other mo(4)C residue forms a wobble pair with the opposite guanine residue. These two pairings are ascribed to the tautomerization of the methoxylated cytosine moieties between the amino and imino forms.
==About this Structure==
==About this Structure==
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1I47 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1I47 OCA].
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1I47 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I47 OCA].
==Reference==
==Reference==
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Crystallographic studies on damaged DNAs: III. N(4)-methoxycytosine can form both Watson-Crick type and wobbled base pairs in a B-form duplex., Hossain MT, Chatake T, Hikima T, Tsunoda M, Sunami T, Ueno Y, Matsuda A, Takenaka A, J Biochem (Tokyo). 2001 Jul;130(1):9-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11432773 11432773]
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Crystallographic studies on damaged DNAs: III. N(4)-methoxycytosine can form both Watson-Crick type and wobbled base pairs in a B-form duplex., Hossain MT, Chatake T, Hikima T, Tsunoda M, Sunami T, Ueno Y, Matsuda A, Takenaka A, J Biochem. 2001 Jul;130(1):9-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11432773 11432773]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Chatake, T.]]
[[Category: Chatake, T.]]
[[Category: Hikima, T.]]
[[Category: Hikima, T.]]
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[[Category: Hossain, M.T.]]
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[[Category: Hossain, M T.]]
[[Category: Matsuda, A.]]
[[Category: Matsuda, A.]]
[[Category: Sunami, T.]]
[[Category: Sunami, T.]]
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[[Category: n4-methoxycytosine]]
[[Category: n4-methoxycytosine]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:35:27 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:07:49 2008''

Revision as of 11:07, 21 February 2008

Image:1i47.gif

1i47, resolution 2.1Å

Drag the structure with the mouse to rotate

MOLECULAR AND CRYSTAL STRUCTURE OF D(CGCGAATT(MO4)CGCG): THE WATSON-CRICK TYPE AND WOBBLE N4-METHOXYCYTIDINE/GUANOSINE BASE PAIRS IN B-DNA

Overview

To investigate the mutation mechanism of purine transition in DNA damaged with methoxyamine, a DNA dodecamer with the sequence d(CGCGAATTmo(4)CGCG), where mo(4)C is 2'-deoxy-N(4)-methoxycytidine, has been synthesized and its crystal structure determined. Two dodecamers form a B-form duplex. Electron density maps clearly show that one of the two mo(4)C residues forms a pair with a guanine residue of the opposite strand, the geometry being the canonical Watson-Crick type, and that the other mo(4)C residue forms a wobble pair with the opposite guanine residue. These two pairings are ascribed to the tautomerization of the methoxylated cytosine moieties between the amino and imino forms.

About this Structure

1I47 is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.

Reference

Crystallographic studies on damaged DNAs: III. N(4)-methoxycytosine can form both Watson-Crick type and wobbled base pairs in a B-form duplex., Hossain MT, Chatake T, Hikima T, Tsunoda M, Sunami T, Ueno Y, Matsuda A, Takenaka A, J Biochem. 2001 Jul;130(1):9-12. PMID:11432773

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