1i9x
From Proteopedia
(New page: 200px<br /><applet load="1i9x" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i9x, resolution 2.18Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1i9x.gif|left|200px]]<br /><applet load="1i9x" size=" | + | [[Image:1i9x.gif|left|200px]]<br /><applet load="1i9x" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1i9x, resolution 2.18Å" /> | caption="1i9x, resolution 2.18Å" /> | ||
'''CRYSTAL STRUCTURE OF BPS-U2 SNRNA DUPLEX'''<br /> | '''CRYSTAL STRUCTURE OF BPS-U2 SNRNA DUPLEX'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Bulged nucleotides play a variety of important roles in RNA structure and | + | Bulged nucleotides play a variety of important roles in RNA structure and function, frequently forming tertiary interactions and sometimes even participating in RNA catalysis. In pre-mRNA splicing, the U2 snRNA base pairs with the intron branchpoint sequence (BPS) to form a short RNA duplex that contains a bulged adenosine that ultimately serves as the nucleophile that attacks the 5' splice site. We have determined a 2.18-A resolution crystal structure of a self-complementary RNA designed to mimic the highly conserved yeast (Saccharomyces cerevisiae) branchpoint sequence (5'-UACUAACGUAGUA with the BPS italicized and the branchsite adenosine underlined) base paired with its complementary sequence from U2 snRNA. The structure shows a nearly ideal A-form helix from which two unpaired adenosines flip out. Although the adenosine adjacent to the branchsite adenosine is the one bulged out in the structure described here, either of these adenosines can serve as the nucleophile in mammalian but not in yeast pre-mRNA splicing. In addition, the packing of the bulged RNA helices within the crystal reveals a novel RNA tertiary interaction in which three RNA helices interact through bulged adenosines in the absence of any divalent metal ions. |
==About this Structure== | ==About this Structure== | ||
| - | 1I9X is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1I9X is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I9X OCA]. |
==Reference== | ==Reference== | ||
Crystal structure of a model branchpoint-U2 snRNA duplex containing bulged adenosines., Berglund JA, Rosbash M, Schultz SC, RNA. 2001 May;7(5):682-91. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11350032 11350032] | Crystal structure of a model branchpoint-U2 snRNA duplex containing bulged adenosines., Berglund JA, Rosbash M, Schultz SC, RNA. 2001 May;7(5):682-91. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11350032 11350032] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Berglund, J | + | [[Category: Berglund, J A.]] |
[[Category: Rosbash, M.]] | [[Category: Rosbash, M.]] | ||
| - | [[Category: Schultz, S | + | [[Category: Schultz, S C.]] |
[[Category: bulged adenosine]] | [[Category: bulged adenosine]] | ||
[[Category: pre-mrna splicing]] | [[Category: pre-mrna splicing]] | ||
[[Category: three helix junction]] | [[Category: three helix junction]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:09:35 2008'' |
Revision as of 11:09, 21 February 2008
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CRYSTAL STRUCTURE OF BPS-U2 SNRNA DUPLEX
Overview
Bulged nucleotides play a variety of important roles in RNA structure and function, frequently forming tertiary interactions and sometimes even participating in RNA catalysis. In pre-mRNA splicing, the U2 snRNA base pairs with the intron branchpoint sequence (BPS) to form a short RNA duplex that contains a bulged adenosine that ultimately serves as the nucleophile that attacks the 5' splice site. We have determined a 2.18-A resolution crystal structure of a self-complementary RNA designed to mimic the highly conserved yeast (Saccharomyces cerevisiae) branchpoint sequence (5'-UACUAACGUAGUA with the BPS italicized and the branchsite adenosine underlined) base paired with its complementary sequence from U2 snRNA. The structure shows a nearly ideal A-form helix from which two unpaired adenosines flip out. Although the adenosine adjacent to the branchsite adenosine is the one bulged out in the structure described here, either of these adenosines can serve as the nucleophile in mammalian but not in yeast pre-mRNA splicing. In addition, the packing of the bulged RNA helices within the crystal reveals a novel RNA tertiary interaction in which three RNA helices interact through bulged adenosines in the absence of any divalent metal ions.
About this Structure
1I9X is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Crystal structure of a model branchpoint-U2 snRNA duplex containing bulged adenosines., Berglund JA, Rosbash M, Schultz SC, RNA. 2001 May;7(5):682-91. PMID:11350032
Page seeded by OCA on Thu Feb 21 13:09:35 2008
