1e15
From Proteopedia
(New page: 200px<br /><applet load="1e15" size="450" color="white" frame="true" align="right" spinBox="true" caption="1e15, resolution 1.90Å" /> '''CHITINASE B FROM SER...) |
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| - | [[Image:1e15.gif|left|200px]]<br /><applet load="1e15" size=" | + | [[Image:1e15.gif|left|200px]]<br /><applet load="1e15" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1e15, resolution 1.90Å" /> | caption="1e15, resolution 1.90Å" /> | ||
'''CHITINASE B FROM SERRATIA MARCESCENS'''<br /> | '''CHITINASE B FROM SERRATIA MARCESCENS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | In this paper, we describe the structure of chitinase B from Serratia | + | In this paper, we describe the structure of chitinase B from Serratia marcescens, which consists of a catalytic domain with a TIM-barrel fold and a 49-residue C-terminal chitin-binding domain. This chitinase is the first structure of a bacterial exochitinase, and it represents one of only a few examples of a glycosyl hydrolase structure having interacting catalytic and substrate-binding domains. The chitin-binding domain has exposed aromatic residues that contribute to a 55-A long continuous aromatic stretch extending into the active site. Binding of chitin oligomers is blocked beyond the -3 subsite, which explains why the enzyme has chitotriosidase activity and degrades the chitin chain from the nonreducing end. Comparison of the chitinase B structure with that of chitinase A explains why these enzymes act synergistically in the degradation of chitin. |
==About this Structure== | ==About this Structure== | ||
| - | 1E15 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]. Full crystallographic information is available from [http:// | + | 1E15 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E15 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Serratia marcescens]] | [[Category: Serratia marcescens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Aalten, D | + | [[Category: Aalten, D M.F Van.]] |
| - | [[Category: Brurberg, M | + | [[Category: Brurberg, M B.]] |
| - | [[Category: Eijsink, V | + | [[Category: Eijsink, V G.H.]] |
[[Category: Hough, E.]] | [[Category: Hough, E.]] | ||
| - | [[Category: Riise, B | + | [[Category: Riise, B W.]] |
[[Category: Synstad, B.]] | [[Category: Synstad, B.]] | ||
| - | [[Category: Wierenga, R | + | [[Category: Wierenga, R K.]] |
[[Category: chitin degradation]] | [[Category: chitin degradation]] | ||
[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:22:42 2008'' |
Revision as of 10:22, 21 February 2008
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CHITINASE B FROM SERRATIA MARCESCENS
Overview
In this paper, we describe the structure of chitinase B from Serratia marcescens, which consists of a catalytic domain with a TIM-barrel fold and a 49-residue C-terminal chitin-binding domain. This chitinase is the first structure of a bacterial exochitinase, and it represents one of only a few examples of a glycosyl hydrolase structure having interacting catalytic and substrate-binding domains. The chitin-binding domain has exposed aromatic residues that contribute to a 55-A long continuous aromatic stretch extending into the active site. Binding of chitin oligomers is blocked beyond the -3 subsite, which explains why the enzyme has chitotriosidase activity and degrades the chitin chain from the nonreducing end. Comparison of the chitinase B structure with that of chitinase A explains why these enzymes act synergistically in the degradation of chitin.
About this Structure
1E15 is a Single protein structure of sequence from Serratia marcescens. Full crystallographic information is available from OCA.
Reference
Structure of a two-domain chitotriosidase from Serratia marcescens at 1.9-A resolution., van Aalten DM, Synstad B, Brurberg MB, Hough E, Riise BW, Eijsink VG, Wierenga RK, Proc Natl Acad Sci U S A. 2000 May 23;97(11):5842-7. PMID:10823940
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