1uhx

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(New page: 200px<br /><applet load="1uhx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1uhx, resolution 2.0&Aring;" /> '''Crystal structure of ...)
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[[Image:1uhx.gif|left|200px]]<br /><applet load="1uhx" size="450" color="white" frame="true" align="right" spinBox="true"
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[[Image:1uhx.gif|left|200px]]<br /><applet load="1uhx" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1uhx, resolution 2.0&Aring;" />
caption="1uhx, resolution 2.0&Aring;" />
'''Crystal structure of d(GCGAGAGC): the base-intercalated duplex'''<br />
'''Crystal structure of d(GCGAGAGC): the base-intercalated duplex'''<br />
==Overview==
==Overview==
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DNA fragments containing the sequence d(GCGAAAGC) prefer to adopt a, base-intercalated (zipper-like) duplex in the crystalline state. To, investigate effects of point mutation at the 5th residue on the structure, two crystal structures of d(GCGAGAGC) and d(GCGATAGC) have been determined, by X-ray crystallography. In the respective crystals, the two octamers, related by a crystallographic two-fold symmetry are aligned in an, anti-parallel fashion and associated to each other to form a duplex, suggesting that the base-intercalated duplex is stable even when the 5th, residue is mutated with other bases. The sheared G3:A6 pair formation, makes the two phosphate backbones closer and facilitates formation of the, A-X*-X-A* base-intercalated motif. The three duplexes are assembled around, the three-fold axis, and their 3rd and 4th residues are bound to the, hexamine cobalt chloride. The central 5th residues are bound to another, cation.
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DNA fragments containing the sequence d(GCGAAAGC) prefer to adopt a base-intercalated (zipper-like) duplex in the crystalline state. To investigate effects of point mutation at the 5th residue on the structure, two crystal structures of d(GCGAGAGC) and d(GCGATAGC) have been determined by X-ray crystallography. In the respective crystals, the two octamers related by a crystallographic two-fold symmetry are aligned in an anti-parallel fashion and associated to each other to form a duplex, suggesting that the base-intercalated duplex is stable even when the 5th residue is mutated with other bases. The sheared G3:A6 pair formation makes the two phosphate backbones closer and facilitates formation of the A-X*-X-A* base-intercalated motif. The three duplexes are assembled around the three-fold axis, and their 3rd and 4th residues are bound to the hexamine cobalt chloride. The central 5th residues are bound to another cation.
==About this Structure==
==About this Structure==
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1UHX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MG, CL, NA and NCO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UHX OCA].
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1UHX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=NCO:'>NCO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UHX OCA].
==Reference==
==Reference==
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[[Category: Sunami, T.]]
[[Category: Sunami, T.]]
[[Category: Takenaka, A.]]
[[Category: Takenaka, A.]]
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[[Category: Umeda, S.I.]]
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[[Category: Umeda, S I.]]
[[Category: CL]]
[[Category: CL]]
[[Category: MG]]
[[Category: MG]]
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[[Category: sheared g:a pair]]
[[Category: sheared g:a pair]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 04:01:19 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:24:40 2008''

Revision as of 13:24, 21 February 2008


1uhx, resolution 2.0Å

Drag the structure with the mouse to rotate

Crystal structure of d(GCGAGAGC): the base-intercalated duplex

Overview

DNA fragments containing the sequence d(GCGAAAGC) prefer to adopt a base-intercalated (zipper-like) duplex in the crystalline state. To investigate effects of point mutation at the 5th residue on the structure, two crystal structures of d(GCGAGAGC) and d(GCGATAGC) have been determined by X-ray crystallography. In the respective crystals, the two octamers related by a crystallographic two-fold symmetry are aligned in an anti-parallel fashion and associated to each other to form a duplex, suggesting that the base-intercalated duplex is stable even when the 5th residue is mutated with other bases. The sheared G3:A6 pair formation makes the two phosphate backbones closer and facilitates formation of the A-X*-X-A* base-intercalated motif. The three duplexes are assembled around the three-fold axis, and their 3rd and 4th residues are bound to the hexamine cobalt chloride. The central 5th residues are bound to another cation.

About this Structure

1UHX is a Protein complex structure of sequences from [1] with , , and as ligands. Full crystallographic information is available from OCA.

Reference

X-ray analyses of d(GCGAXAGC) containing G and T at X: the base-intercalated duplex is still stable even in point mutants at the fifth residue., Kondo J, Umeda S, Fujita K, Sunami T, Takenaka A, J Synchrotron Radiat. 2004 Jan 1;11(Pt 1):117-20. Epub 2003 Nov 28. PMID:14646150

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