1ukg
From Proteopedia
(New page: 200px<br /><applet load="1ukg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ukg, resolution 1.7Å" /> '''Pterocarps angolensis...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1ukg.jpg|left|200px]]<br /><applet load="1ukg" size=" | + | [[Image:1ukg.jpg|left|200px]]<br /><applet load="1ukg" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ukg, resolution 1.7Å" /> | caption="1ukg, resolution 1.7Å" /> | ||
'''Pterocarps angolensis lectin PAL in complex with methyl-alpha-mannose'''<br /> | '''Pterocarps angolensis lectin PAL in complex with methyl-alpha-mannose'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of a Man/Glc-specific lectin from the seeds of the | + | The crystal structure of a Man/Glc-specific lectin from the seeds of the bloodwood tree (Pterocarpus angolensis), a leguminous plant from central Africa, has been determined in complex with mannose and five manno-oligosaccharides. The lectin contains a classical mannose-specificity loop, but its metal-binding loop resembles that of lectins of unrelated specificity from Ulex europaeus and Maackia amurensis. As a consequence, the interactions with mannose in the primary binding site are conserved, but details of carbohydrate-binding outside the primary binding site differ from those seen in the equivalent carbohydrate complexes of concanavalin A. These observations explain the differences in their respective fine specificity profiles for oligomannoses. While Man(alpha1-3)Man and Man(alpha1-3)[Man(alpha1-6)]Man bind to PAL in low-energy conformations identical with that of ConA, Man(alpha1-6)Man is required to adopt a different conformation. Man(alpha1-2)Man can bind only in a single binding mode, in sharp contrast to ConA, which creates a higher affinity for this disaccharide by allowing two binding modes. |
==About this Structure== | ==About this Structure== | ||
| - | 1UKG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pterocarpus_angolensis Pterocarpus angolensis] with MMA, MN and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1UKG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pterocarpus_angolensis Pterocarpus angolensis] with <scene name='pdbligand=MMA:'>MMA</scene>, <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UKG OCA]. |
==Reference== | ==Reference== | ||
| Line 16: | Line 16: | ||
[[Category: Bouckaert, J.]] | [[Category: Bouckaert, J.]] | ||
[[Category: Deboeck, F.]] | [[Category: Deboeck, F.]] | ||
| - | [[Category: Greve, H | + | [[Category: Greve, H De.]] |
[[Category: Loris, R.]] | [[Category: Loris, R.]] | ||
| - | [[Category: Walle, I | + | [[Category: Walle, I Van.]] |
[[Category: Wyns, L.]] | [[Category: Wyns, L.]] | ||
[[Category: CA]] | [[Category: CA]] | ||
| Line 27: | Line 27: | ||
[[Category: mannose]] | [[Category: mannose]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:25:29 2008'' |
Revision as of 13:25, 21 February 2008
|
Pterocarps angolensis lectin PAL in complex with methyl-alpha-mannose
Overview
The crystal structure of a Man/Glc-specific lectin from the seeds of the bloodwood tree (Pterocarpus angolensis), a leguminous plant from central Africa, has been determined in complex with mannose and five manno-oligosaccharides. The lectin contains a classical mannose-specificity loop, but its metal-binding loop resembles that of lectins of unrelated specificity from Ulex europaeus and Maackia amurensis. As a consequence, the interactions with mannose in the primary binding site are conserved, but details of carbohydrate-binding outside the primary binding site differ from those seen in the equivalent carbohydrate complexes of concanavalin A. These observations explain the differences in their respective fine specificity profiles for oligomannoses. While Man(alpha1-3)Man and Man(alpha1-3)[Man(alpha1-6)]Man bind to PAL in low-energy conformations identical with that of ConA, Man(alpha1-6)Man is required to adopt a different conformation. Man(alpha1-2)Man can bind only in a single binding mode, in sharp contrast to ConA, which creates a higher affinity for this disaccharide by allowing two binding modes.
About this Structure
1UKG is a Single protein structure of sequence from Pterocarpus angolensis with , and as ligands. Full crystallographic information is available from OCA.
Reference
Structural basis of oligomannose recognition by the Pterocarpus angolensis seed lectin., Loris R, Van Walle I, De Greve H, Beeckmans S, Deboeck F, Wyns L, Bouckaert J, J Mol Biol. 2004 Jan 30;335(5):1227-40. PMID:14729339
Page seeded by OCA on Thu Feb 21 15:25:29 2008
Categories: Pterocarpus angolensis | Single protein | Beeckmans, S. | Bouckaert, J. | Deboeck, F. | Greve, H De. | Loris, R. | Walle, I Van. | Wyns, L. | CA | MMA | MN | Carbohydrate | Lectin | Mannose
