1mfy
From Proteopedia
(New page: 200px<br /><applet load="1mfy" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mfy" /> '''SOLUTION STRUCTURE OF INFLUENZA A VIRUS C4 P...) |
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| - | [[Image:1mfy.gif|left|200px]]<br /><applet load="1mfy" size=" | + | [[Image:1mfy.gif|left|200px]]<br /><applet load="1mfy" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1mfy" /> | caption="1mfy" /> | ||
'''SOLUTION STRUCTURE OF INFLUENZA A VIRUS C4 PROMOTER'''<br /> | '''SOLUTION STRUCTURE OF INFLUENZA A VIRUS C4 PROMOTER'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The influenza A virus promoter is recognized by the influenza A virus | + | The influenza A virus promoter is recognized by the influenza A virus RNA-dependent RNA polymerase, and directs both transcription and replication of the viral RNA genome. Within the sequence of this promoter, flu strains exhibit a natural, unique variation, either a U or a C, at the fourth position from the 3' end. Promoters that contain a C residue (C4 promoter), which are invariably found in genome segments that encode the three RNA polymerase subunits (PB1, PB2 and PA), down-regulate transcription but activate genome replication. Here, we have determined the structure of the C4 promoter by NMR spectroscopy and compared it with the structure of the U4 promoter, which was determined previously. The structure of the internal loop in the C4 promoter is similar to that of the U4 promoter. However, the terminal stem of the C4 promoter is strikingly different from that of the U4 promoter. These structural data suggest that the internal loop is important for polymerase binding to the promoter, and the terminal stem is crucial for differential regulation of transcription and replication. |
==About this Structure== | ==About this Structure== | ||
| - | 1MFY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1MFY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MFY OCA]. |
==Reference== | ==Reference== | ||
A single-nucleotide natural variation (U4 to C4) in an influenza A virus promoter exhibits a large structural change: implications for differential viral RNA synthesis by RNA-dependent RNA polymerase., Lee MK, Bae SH, Park CJ, Cheong HK, Cheong C, Choi BS, Nucleic Acids Res. 2003 Feb 15;31(4):1216-23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12582241 12582241] | A single-nucleotide natural variation (U4 to C4) in an influenza A virus promoter exhibits a large structural change: implications for differential viral RNA synthesis by RNA-dependent RNA polymerase., Lee MK, Bae SH, Park CJ, Cheong HK, Cheong C, Choi BS, Nucleic Acids Res. 2003 Feb 15;31(4):1216-23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12582241 12582241] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Bae, S | + | [[Category: Bae, S H.]] |
[[Category: Cheong, C.]] | [[Category: Cheong, C.]] | ||
| - | [[Category: Cheong, H | + | [[Category: Cheong, H K.]] |
| - | [[Category: Choi, B | + | [[Category: Choi, B S.]] |
| - | [[Category: Lee, M | + | [[Category: Lee, M K.]] |
| - | [[Category: Park, C | + | [[Category: Park, C J.]] |
[[Category: c4]] | [[Category: c4]] | ||
[[Category: influenza a virus promoter]] | [[Category: influenza a virus promoter]] | ||
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[[Category: single adenine bulge]] | [[Category: single adenine bulge]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:54:50 2008'' |
Revision as of 11:54, 21 February 2008
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SOLUTION STRUCTURE OF INFLUENZA A VIRUS C4 PROMOTER
Overview
The influenza A virus promoter is recognized by the influenza A virus RNA-dependent RNA polymerase, and directs both transcription and replication of the viral RNA genome. Within the sequence of this promoter, flu strains exhibit a natural, unique variation, either a U or a C, at the fourth position from the 3' end. Promoters that contain a C residue (C4 promoter), which are invariably found in genome segments that encode the three RNA polymerase subunits (PB1, PB2 and PA), down-regulate transcription but activate genome replication. Here, we have determined the structure of the C4 promoter by NMR spectroscopy and compared it with the structure of the U4 promoter, which was determined previously. The structure of the internal loop in the C4 promoter is similar to that of the U4 promoter. However, the terminal stem of the C4 promoter is strikingly different from that of the U4 promoter. These structural data suggest that the internal loop is important for polymerase binding to the promoter, and the terminal stem is crucial for differential regulation of transcription and replication.
About this Structure
1MFY is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
A single-nucleotide natural variation (U4 to C4) in an influenza A virus promoter exhibits a large structural change: implications for differential viral RNA synthesis by RNA-dependent RNA polymerase., Lee MK, Bae SH, Park CJ, Cheong HK, Cheong C, Choi BS, Nucleic Acids Res. 2003 Feb 15;31(4):1216-23. PMID:12582241
Page seeded by OCA on Thu Feb 21 13:54:50 2008
