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User:Amy Kerzmann/Sandbox 5

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<center>'''Elastase''', resolution 1.78 &Aring; (<scene name='User:Amy_Kerzmann/Sandbox_5/Elastase/2'>initial scene</scene>).</center><applet load='4est' size='300' frame='true' align='right' scene='User:Amy_Kerzmann/Sandbox_5/Elastase/2' /></td><td bgcolor='#eeeeee'></td></table>
<center>'''Elastase''', resolution 1.78 &Aring; (<scene name='User:Amy_Kerzmann/Sandbox_5/Elastase/2'>initial scene</scene>).</center><applet load='4est' size='300' frame='true' align='right' scene='User:Amy_Kerzmann/Sandbox_5/Elastase/2' /></td><td bgcolor='#eeeeee'></td></table>
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Serine proteases perform their catalytic roles using three key residues: Ser, Asp, His. Because the proteases utilize these three residues for function, they are referred to as the '''catalytic triad'''. Highlight the <scene name='User:Amy_Kerzmann/Sandbox_5/Chymotrypsin-triad/2' target='0'>chymotrypsin catalytic triad</scene>. Mouse over the structure to determine the residue numbers for Ser, Asp and His.
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Serine proteases perform their catalytic roles using three key residues: Ser, His, Asp. Because the proteases utilize these three residues for function, they are referred to as the '''catalytic triad'''. Highlight the <scene name='User:Amy_Kerzmann/Sandbox_5/Chymotrypsin-triad/2' target='0'>chymotrypsin catalytic triad</scene>. Mouse over the structure to determine the residue numbers for Ser, Asp and His.
Now compare chymotrypsin to <scene name='User:Amy_Kerzmann/Sandbox_5/Trypsin-wt-triad/1' target='1'>trypsin catalytic triad</scene>. The <scene name='User:Amy_Kerzmann/Sandbox_5/Elastase-triad/2' target='2'>elastase catalytic triad</scene>.
Now compare chymotrypsin to <scene name='User:Amy_Kerzmann/Sandbox_5/Trypsin-wt-triad/1' target='1'>trypsin catalytic triad</scene>. The <scene name='User:Amy_Kerzmann/Sandbox_5/Elastase-triad/2' target='2'>elastase catalytic triad</scene>.
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{
{
|type="{}"}
|type="{}"}
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What are the residue numbers of the catalytic triad? Provide in the order shown: Ser, Asp, then His.
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What are the residue numbers of the catalytic triad? Provide in the order shown: Ser, His, then Asp.
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{ 195, 102, 57|195, 102, and 57 }
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{ 195, 57, 102|195, 57 and 102 }
{Which of the following statements is false?
{Which of the following statements is false?

Revision as of 08:17, 10 February 2010

Serine Proteases

Family includes trypsin, chymotrypsin and elastase.


 
Chymotrypsin, resolution 2.00 Å ().

PDB ID 2cha

Drag the structure with the mouse to rotate
Trypsin, resolution 1.34 Å ().

PDB ID 1aq7

Drag the structure with the mouse to rotate
Elastase, resolution 1.78 Å ().

PDB ID 4est

Drag the structure with the mouse to rotate

Serine proteases perform their catalytic roles using three key residues: Ser, His, Asp. Because the proteases utilize these three residues for function, they are referred to as the catalytic triad. Highlight the . Mouse over the structure to determine the residue numbers for Ser, Asp and His.

Now compare chymotrypsin to . The .

1.

What are the residue numbers of the catalytic triad? Provide in the order shown: Ser, His, then Asp.

2. Which of the following statements is false?

Trypsin contains an Asp residue, which selects for Arg and Lys substrates.
Chymotrypsin has a large deep binding pocket.
Elastase has two valines that provide steric hinderance.
None of the above; they are all true.

Your score is 0 / 0

Proteopedia Page Contributors and Editors (what is this?)

Amy Kerzmann, Eric Martz

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