2pc6

From Proteopedia

Revision as of 10:50, 23 January 2008

Crystal structure of putative acetolactate synthase- small subunit from Nitrosomonas europaea

Overview

Crystal structures of two orthologs of the regulatory subunit of, acetohydroxyacid synthase III (AHAS, EC 2.2.1.6) from Thermotoga maritima, (TM0549) and Nitrosomonas europea (NE1324) were determined by, single-wavelength anomalous diffraction methods with the use of, selenomethionine derivatives at 2.3 A and 2.5 A, respectively. TM0549 and, NE1324 share the same fold, and in both proteins the polypeptide chain, contains two separate domains of a similar size. Each protein contains a, C-terminal domain with ferredoxin-type fold and an N-terminal ACT domain, of which the latter is characteristic for several proteins involved in, amino acid metabolism. The ferredoxin domain is stabilized by a calcium, ion in the crystal structure of NE1324 and by a Mg(H2O)(6)2+ ion in, TM0549. Both TM0549 and NE1324 form dimeric assemblies in the crystal, lattice.

About this Structure

2PC6 is a Single protein structure of sequence from Nitrosomonas europaea atcc 19718 with and as ligands. This structure superseeds the now removed PDB entry 2FGD. Active as Acetolactate synthase, with EC number 2.2.1.6 Full crystallographic information is available from OCA.

Reference

Crystal structures of TM0549 and NE1324--two orthologs of E. coli AHAS isozyme III small regulatory subunit., Petkowski JJ, Chruszcz M, Zimmerman MD, Zheng H, Skarina T, Onopriyenko O, Cymborowski MT, Koclega KD, Savchenko A, Edwards A, Minor W, Protein Sci. 2007 Jul;16(7):1360-7. PMID:17586771

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