1pfd
From Proteopedia
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Revision as of 13:54, 30 October 2007
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THE SOLUTION STRUCTURE OF HIGH PLANT PARSLEY [2FE-2S] FERREDOXIN, NMR, 18 STRUCTURES
Overview
The [2Fe-2S]ferredoxin I (Fd I) from parsley leaves (Mr = 10,500; 96 amino, acids) in the Fe(III)-Fe(III) oxidized form has been studied by 1H-NMR, spectroscopy. Sequence-specific 1H-NMR assignments were obtained through, two-dimensional classical double-quantum-filtered-COSY, NOESY and TOCSY, spectra. NOEs between protons as close as 5.6 A from the paramagnetic, Fe(III) atoms were observed at 800 MHz. A total of 3066 NOEs (of which, 2533 are meaningful) and 18 distance constraints taken from X-ray, crystallography of the Fe2S2 active site were used to obtain the solution, structure. From inversion recovery NOESY experiments, 33 longitudinal, relaxation rate (Qpara) constraints were used for the structural, refinement. The final structure was obtained by a process of restrained, energy ... [(full description)]
About this Structure
1PFD is a [Single protein] structure of sequence from [Petroselinum crispum] with FES as [ligand]. Active as [Ferredoxin--nitrate reductase], with EC number [1.7.7.2]. Structure known Active Site: FES. Full crystallographic information is available from [OCA].
Reference
The solution structure of parsley [2Fe-2S]ferredoxin., Im SC, Liu G, Luchinat C, Sykes AG, Bertini I, Eur J Biochem. 1998 Dec 1;258(2):465-77. PMID:9874213[[Category: [2fe-2s] ferredoxin]]
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