1wcg

From Proteopedia

Revision as of 14:26, 30 October 2007

APHID MYROSINASE

Overview

The aphid Brevicoryne brassicae is a specialist feeding on Brassicaceae, plants. The insect has an intricate defence system involving a, beta-D-thioglucosidase (myrosinase) that hydrolyses glucosinolates, sequestered from the host plant into volatile isothiocyanates. These, isothiocyanates act synergistically with the pheromone E-beta-farnesene to, form an alarm system when the aphid is predated. In order to investigate, the enzymatic characteristics of the aphid myrosinase and its, three-dimensional structure, milligram amounts of pure recombinant aphid, myrosinase were obtained from Echerichia coli. The recombinant enzyme had, similar physiochemical properties to the native enzyme. The global, structure is very similar to Sinapis alba myrosinase and plant, beta-O-glucosidases. Aphid ... [(full description)]

About this Structure

1WCG is a [Single protein] structure of sequence from [Brevicoryne brassicae] with GOL as [ligand]. Active as [Transferred entry: 3.2.1.147], with EC number [3.2.3.1]. Structure known Active Site: GLA. Full crystallographic information is available from [OCA].

Reference

Crystal structure at 1.1 Angstroms resolution of an insect myrosinase from Brevicoryne brassicae shows its close relationship to beta-glucosidases., Husebye H, Arzt S, Burmeister WP, Hartel FV, Brandt A, Rossiter JT, Bones AM, Insect Biochem Mol Biol. 2005 Dec;35(12):1311-20. Epub 2005 Aug 18. PMID:16291087

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