1at5
From Proteopedia
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| - | [[Image:1at5.gif|left|200px]]<br /><applet load="1at5" size=" | + | [[Image:1at5.gif|left|200px]]<br /><applet load="1at5" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1at5, resolution 1.80Å" /> | caption="1at5, resolution 1.80Å" /> | ||
'''HEN EGG WHITE LYSOZYME WITH A SUCCINIMIDE RESIDUE'''<br /> | '''HEN EGG WHITE LYSOZYME WITH A SUCCINIMIDE RESIDUE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1AT5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with NA and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Known structural/functional Site: <scene name='pdbsite=SUC:Succinimide Is Formed At ASP 101 - GLY 102'>SUC</scene>. Full crystallographic information is available from [http:// | + | 1AT5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Known structural/functional Site: <scene name='pdbsite=SUC:Succinimide+Is+Formed+At+ASP+101+-+GLY+102'>SUC</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AT5 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: succinimide]] | [[Category: succinimide]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:31:31 2008'' |
Revision as of 07:31, 3 February 2008
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HEN EGG WHITE LYSOZYME WITH A SUCCINIMIDE RESIDUE
Overview
The isomerization of Asp101 to isoaspartate autocatalytically proceeds via, a succinimide intermediate in hen egg-white lysozyme at a mildly acidic, condition. The crystal structures of succinimide and isoaspartate forms of, the lysozyme proteins, each complexed with a tri-N-acetylchitotriose, ligand, have been determined at 1.8 A resolution, and distinctively, elucidate coplanar cyclic aminosuccinyl and beta-linked isoaspartyl, residues. Compared with the liganded native protein with normal Asp101, succinimide 101 protrudes toward the ligand, and isoaspartate 101 extends, away from the ligand. The formations of these residues caused the loss of, three hydrogen-bonds between the ligand and the side-chains of Asp101 and, Asn103 along with 0.5 A displacement of the ligand location.
About this Structure
1AT5 is a Single protein structure of sequence from Gallus gallus with and as ligands. Active as Lysozyme, with EC number 3.2.1.17 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Succinimide and isoaspartate residues in the crystal structures of hen egg-white lysozyme complexed with tri-N-acetylchitotriose., Noguchi S, Miyawaki K, Satow Y, J Mol Biol. 1998 Apr 24;278(1):231-8. PMID:9571046
Page seeded by OCA on Sun Feb 3 09:31:31 2008
Categories: Gallus gallus | Lysozyme | Single protein | Miyawaki, K. | Noguchi, S. | Satow, Y. | CL | NA | Hydrolase | O-glycosyl hydrolase | Succinimide
