1brt
From Proteopedia
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| - | [[Image:1brt.jpg|left|200px]]<br /><applet load="1brt" size=" | + | [[Image:1brt.jpg|left|200px]]<br /><applet load="1brt" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1brt, resolution 1.50Å" /> | caption="1brt, resolution 1.50Å" /> | ||
'''BROMOPEROXIDASE A2 MUTANT M99T'''<br /> | '''BROMOPEROXIDASE A2 MUTANT M99T'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1BRT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_aureofaciens Streptomyces aureofaciens] with CL as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Chloride_peroxidase Chloride peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.10 1.11.1.10] Known structural/functional Site: <scene name='pdbsite=NUL:Catalytic Triad'>NUL</scene>. Full crystallographic information is available from [http:// | + | 1BRT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_aureofaciens Streptomyces aureofaciens] with <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Chloride_peroxidase Chloride peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.10 1.11.1.10] Known structural/functional Site: <scene name='pdbsite=NUL:Catalytic+Triad'>NUL</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BRT OCA]. |
==Reference== | ==Reference== | ||
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[[Category: peroxidase]] | [[Category: peroxidase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:33:44 2008'' |
Revision as of 07:33, 3 February 2008
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BROMOPEROXIDASE A2 MUTANT M99T
Overview
The structures of cofactor-free haloperoxidases from Streptomyces, aureofaciens, Streptomyces lividans, and Pseudomonas fluorescens have been, determined at resolutions between 1.9 A and 1.5 A. The structures of two, enzymes complexed with benzoate or propionate identify the binding site, for the organic acids which are required for the haloperoxidase activity., Based on these complexes and on the structure of an inactive variant, a, reaction mechanism is proposed for the halogenation reaction with, peroxoacid and hypohalous acid as reaction intermediates. Comparison of, the structures suggests that a specific halide binding site is absent in, the enzymes but that hydrophobic organic compounds may fit into the active, site pocket for halogenation at preferential sites.
About this Structure
1BRT is a Single protein structure of sequence from Streptomyces aureofaciens with as ligand. Active as Chloride peroxidase, with EC number 1.11.1.10 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structural investigation of the cofactor-free chloroperoxidases., Hofmann B, Tolzer S, Pelletier I, Altenbuchner J, van Pee KH, Hecht HJ, J Mol Biol. 1998 Jun 19;279(4):889-900. PMID:9642069
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