1bzf
From Proteopedia
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| - | [[Image:1bzf.gif|left|200px]]<br /><applet load="1bzf" size=" | + | [[Image:1bzf.gif|left|200px]]<br /><applet load="1bzf" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1bzf" /> | caption="1bzf" /> | ||
'''NMR SOLUTION STRUCTURE AND DYNAMICS OF THE COMPLEX OF LACTOBACILLUS CASEI DIHYDROFOLATE REDUCTASE WITH THE NEW LIPOPHILIC ANTIFOLATE DRUG TRIMETREXATE, 22 STRUCTURES'''<br /> | '''NMR SOLUTION STRUCTURE AND DYNAMICS OF THE COMPLEX OF LACTOBACILLUS CASEI DIHYDROFOLATE REDUCTASE WITH THE NEW LIPOPHILIC ANTIFOLATE DRUG TRIMETREXATE, 22 STRUCTURES'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1BZF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lactobacillus_casei Lactobacillus casei] with TMQ as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] Known structural/functional Site: <scene name='pdbsite=TMQ:Residues w. Observed Noes To Bound Trimetrexate'>TMQ</scene>. Full crystallographic information is available from [http:// | + | 1BZF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lactobacillus_casei Lactobacillus casei] with <scene name='pdbligand=TMQ:'>TMQ</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] Known structural/functional Site: <scene name='pdbsite=TMQ:Residues+w.+Observed+Noes+To+Bound+Trimetrexate'>TMQ</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BZF OCA]. |
==Reference== | ==Reference== | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:34:20 2008'' |
Revision as of 07:34, 3 February 2008
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NMR SOLUTION STRUCTURE AND DYNAMICS OF THE COMPLEX OF LACTOBACILLUS CASEI DIHYDROFOLATE REDUCTASE WITH THE NEW LIPOPHILIC ANTIFOLATE DRUG TRIMETREXATE, 22 STRUCTURES
Overview
We have determined the three-dimensional solution structure of the complex, of Lactobacillus casei dihydrofolate reductase and the anticancer drug, trimetrexate. Two thousand seventy distance, 345 dihedral angle, and 144, hydrogen bond restraints were obtained from analysis of multidimensional, NMR spectra recorded for complexes containing 15N-labeled protein., Simulated annealing calculations produced a family of 22 structures fully, consistent with the constraints. Several intermolecular protein-ligand, NOEs were obtained by using a novel approach monitoring temperature, effects of NOE signals resulting from dynamic processes in the bound, ligand. At low temperature (5 degrees C) the trimethoxy ring of bound, trimetrexate is flipping sufficiently slowly to give narrow signals in, slow exchange, which give good NOE cross peaks. At higher temperature, these broaden and their NOE cross peaks disappear thus allowing the, signals in the lower-temperature spectrum to be identified as NOEs, involving ligand protons. The binding site for trimetrexate is well, defined and this was compared with the binding sites in related complexes, formed with methotrexate and trimethoprim. No major conformational, differences were detected between the different complexes. The, 2,4-diaminopyrimidine-containing moieties in the three drugs bind, essentially in the same binding pocket and the remaining parts of their, molecules adapt their conformations such that they can make effective van, der Waals interactions with essentially the same set of hydrophobic amino, acids, the side-chain orientations and local conformations of which are, not greatly changed in the different complexes (similar chi1 and chi2, values).
About this Structure
1BZF is a Single protein structure of sequence from Lactobacillus casei with as ligand. Active as Dihydrofolate reductase, with EC number 1.5.1.3 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structure and dynamics in solution of the complex of Lactobacillus casei dihydrofolate reductase with the new lipophilic antifolate drug trimetrexate., Polshakov VI, Birdsall B, Frenkiel TA, Gargaro AR, Feeney J, Protein Sci. 1999 Mar;8(3):467-81. PMID:10091649
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