1eb6
From Proteopedia
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| - | [[Image:1eb6.jpg|left|200px]]<br /><applet load="1eb6" size=" | + | [[Image:1eb6.jpg|left|200px]]<br /><applet load="1eb6" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1eb6, resolution 1.00Å" /> | caption="1eb6, resolution 1.00Å" /> | ||
'''DEUTEROLYSIN FROM ASPERGILLUS ORYZAE'''<br /> | '''DEUTEROLYSIN FROM ASPERGILLUS ORYZAE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1EB6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae] with ZN and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Deuterolysin Deuterolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.39 3.4.24.39] Known structural/functional Site: <scene name='pdbsite=ZN:Zn Binding Site'>ZN</scene>. Full crystallographic information is available from [http:// | + | 1EB6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Deuterolysin Deuterolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.39 3.4.24.39] Known structural/functional Site: <scene name='pdbsite=ZN:Zn+Binding+Site'>ZN</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EB6 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: zinc]] | [[Category: zinc]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:38:57 2008'' |
Revision as of 07:38, 3 February 2008
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DEUTEROLYSIN FROM ASPERGILLUS ORYZAE
Overview
A data set from the metalloproteinase deuterolysin was collected at atomic, resolution (1.0 A) with synchrotron radiation. The high resolution allowed, the structure to be solved with the new direct-methods program ACORN using, the coordinates of the Zn atom as a starting point. The phases obtained, from ACORN were of sufficient quality to allow automated building to be, carried out in ARP/wARP. Minimal manual rebuilding of the model was, required and the structure determination was completed using the, maximum-likelihood refinement program REFMAC. The whole process, starting, from the processed and merged data and ending with a refined model, required less than 6 h of computational time.
About this Structure
1EB6 is a Single protein structure of sequence from Aspergillus oryzae with and as ligands. Active as Deuterolysin, with EC number 3.4.24.39 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
A quick solution: ab initio structure determination of a 19 kDa metalloproteinase using ACORN., McAuley KE, Jia-Xing Y, Dodson EJ, Lehmbeck J, Ostergaard PR, Wilson KS, Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1571-8. Epub 2001, Oct 25. PMID:11679721
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