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1fdo
From Proteopedia
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| - | [[Image:1fdo.jpg|left|200px]]<br /><applet load="1fdo" size=" | + | [[Image:1fdo.jpg|left|200px]]<br /><applet load="1fdo" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1fdo, resolution 2.8Å" /> | caption="1fdo, resolution 2.8Å" /> | ||
'''OXIDIZED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI'''<br /> | '''OXIDIZED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1FDO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SF4, MGD and 6MO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Formate_dehydrogenase Formate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.2 1.2.1.2] Known structural/functional Sites: <scene name='pdbsite=FS4:The Fe S Cluster Is Coordinated To The S Atoms Of CYS 8, ...'>FS4</scene> and <scene name='pdbsite=MO4:The Mo Atom Is Coordinated To The Se Atom Of Sec 140, Th ...'>MO4</scene>. Full crystallographic information is available from [http:// | + | 1FDO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=SF4:'>SF4</scene>, <scene name='pdbligand=MGD:'>MGD</scene> and <scene name='pdbligand=6MO:'>6MO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Formate_dehydrogenase Formate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.2 1.2.1.2] Known structural/functional Sites: <scene name='pdbsite=FS4:The+Fe+S+Cluster+Is+Coordinated+To+The+S+Atoms+Of+CYS+8,+...'>FS4</scene> and <scene name='pdbsite=MO4:The+Mo+Atom+Is+Coordinated+To+The+Se+Atom+Of+Sec+140,+Th+...'>MO4</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FDO OCA]. |
==Reference== | ==Reference== | ||
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[[Category: selenocysteine]] | [[Category: selenocysteine]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:39:43 2008'' |
Revision as of 07:39, 3 February 2008
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OXIDIZED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI
Overview
Formate dehydrogenase H from Escherichia coli contains selenocysteine, (SeCys), molybdenum, two molybdopterin guanine dinucleotide (MGD), cofactors, and an Fe4S4 cluster at the active site and catalyzes the, two-electron oxidation of formate to carbon dioxide. The crystal, structures of the oxidized [Mo(VI), Fe4S4(ox)] form of formate, dehydrogenase H (with and without bound inhibitor) and the reduced, [Mo(IV), Fe4S4(red)] form have been determined, revealing a four-domain, alphabeta structure with the molybdenum directly coordinated to selenium, and both MGD cofactors. These structures suggest a reaction mechanism that, directly involves SeCys140 and His141 in proton abstraction and the, molybdenum, molybdopterin, Lys44, and the Fe4S4 cluster in electron, transfer.
About this Structure
1FDO is a Single protein structure of sequence from Escherichia coli with , and as ligands. Active as Formate dehydrogenase, with EC number 1.2.1.2 Known structural/functional Sites: and . Full crystallographic information is available from OCA.
Reference
Crystal structure of formate dehydrogenase H: catalysis involving Mo, molybdopterin, selenocysteine, and an Fe4S4 cluster., Boyington JC, Gladyshev VN, Khangulov SV, Stadtman TC, Sun PD, Science. 1997 Feb 28;275(5304):1305-8. PMID:9036855
Page seeded by OCA on Sun Feb 3 09:39:43 2008
Categories: Escherichia coli | Formate dehydrogenase | Single protein | Boyington, J.C. | Sun, P.D. | 6MO | MGD | SF4 | Anaerobic | Dehydrogenase | Fe4s4 | Formate | Iron sulfur cluster | Mgd | Molybdenum | Molybdopterin | Molybdopterin guanine dinucleotide | Mpt | Oxidoreductase | Secys | Selenium | Selenocysteine
