Sonic Hedgehog
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= Introduction = | = Introduction = | ||
- | Sonic hedgehog (Shh) is a member of the Hedgehog (Hh) family of secreted extracellular signaling proteins, which serve important roles in regulating both short-range and long-range patterning processes in developing invertebrate and vertebrate tissues<ref>PMID: 7867057</ref>. First discovered in ''Drosophila'', where mutations of the single ''Hedgehog'' gene produces larvae that are covered in hedgehog-like denticles, Hh proteins are encoded by at least three genes in mammals - ''Sonic'', ''Desert'', and ''Indian hedgehog''<ref>PMID: 7916661</ref>. With the ability to control such fundamental processes as the anterioposterior patterning of vertebrate limb buds<ref name="limb">PMID: 8269518</ref>, the formation of motor neurons in the neural tube <ref name="neuron">PMID: 7736596</ref>, and the development and maintenance of tissues and organs<ref>PMID: 10980429</ref>, Shh is the most well-studied member of the Hh signaling proteins<ref name="papinsky">PMID: 10753901</ref>. Excessive signaling in adult cells has been implicated in the development of several human cancers<ref>PMID: 14737121</ref><ref name="Path">PMID: 12044012</ref>. Mutations in the ''Shh'' gene can also result in severe developmental consequences, including [http://en.wikipedia.org/wiki/Holoprosencephaly holoprosencephaly]. | + | Sonic hedgehog (Shh) is a member of the Hedgehog (Hh) family of secreted extracellular signaling proteins, which serve important roles in regulating both short-range and long-range patterning processes in developing invertebrate and vertebrate tissues<ref>PMID: 7867057</ref>. First discovered in ''Drosophila'', where mutations of the single ''Hedgehog'' gene produces larvae that are covered in hedgehog-like denticles, Hh proteins are encoded by at least three genes in mammals - ''Sonic'', ''Desert'', and ''Indian hedgehog''<ref>PMID: 7916661</ref>. With the ability to control such fundamental processes as the anterioposterior patterning of vertebrate limb buds<ref name="limb">PMID: 8269518</ref>, the formation of motor neurons in the neural tube <ref name="neuron">PMID: 7736596</ref>, and the development and maintenance of tissues and organs<ref>PMID: 10980429</ref>, Shh is the most well-studied member of the Hh signaling proteins<ref name="papinsky">PMID: 10753901</ref>. Excessive signaling in adult cells has been implicated in the development of several human cancers<ref>PMID: 14737121</ref><ref name="Path">PMID: 12044012</ref>. Mutations in the ''Shh'' gene can also result in severe developmental consequences, including [http://en.wikipedia.org/wiki/Holoprosencephaly holoprosencephaly] <ref>PMID: 8896572</ref>. |
= Biosynthesis = | = Biosynthesis = |
Revision as of 17:10, 31 March 2010
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Contents |
Introduction
Sonic hedgehog (Shh) is a member of the Hedgehog (Hh) family of secreted extracellular signaling proteins, which serve important roles in regulating both short-range and long-range patterning processes in developing invertebrate and vertebrate tissues[1]. First discovered in Drosophila, where mutations of the single Hedgehog gene produces larvae that are covered in hedgehog-like denticles, Hh proteins are encoded by at least three genes in mammals - Sonic, Desert, and Indian hedgehog[2]. With the ability to control such fundamental processes as the anterioposterior patterning of vertebrate limb buds[3], the formation of motor neurons in the neural tube [4], and the development and maintenance of tissues and organs[5], Shh is the most well-studied member of the Hh signaling proteins[6]. Excessive signaling in adult cells has been implicated in the development of several human cancers[7][8]. Mutations in the Shh gene can also result in severe developmental consequences, including holoprosencephaly [9].
Biosynthesis
As with all members of the Hh family, Shh biosynthesis begins with an unusual molecular processing event. Following cleavage of its signal peptide, the Shh precursor protein is autocatalytically cleaved into two functionally distinct domains, a 19-kDa amino-terminal domain (Shh-N) and a 27-kDa carboxy-terminal domain (Shh-C)[10]. Spanning residues 24 to 197 in human Shh, Shh-N is responsible for all of the local and long-range signaling activities of Shh. Shh-C possesses an intramolecular transferase activity responsible for covalent attachment of a molecule of cholesterol to the C-terminus of Shh-N [6]. The addition of cholesterol serves to tether Shh-N to the cell membrane, restricting its range of activity to that of local signaling only[11]. A second modification involving the attachment of a palmitoyl group to Cys-24 on the protein's N-terminus has recently been discovered in insect and mammalian cells. This N-terminal modification is thought to increase the potency of the Shh-N signal as much as 30-fold[12].
Structural Overview

The crystal structure of Shh-N also contains a single sulphate molecule, shown in red and white, which interacts with arginine residues at the interface of two Shh-N molecules. Since Shh-N binds heparin, this interaction is believed to comprise a heparin-binding region[13].
Function
Sonic Signaling: The Shh-Gli Pathway
References
- ↑ Perrimon N. Hedgehog and beyond. Cell. 1995 Feb 24;80(4):517-20. PMID:7867057
- ↑ Echelard Y, Epstein DJ, St-Jacques B, Shen L, Mohler J, McMahon JA, McMahon AP. Sonic hedgehog, a member of a family of putative signaling molecules, is implicated in the regulation of CNS polarity. Cell. 1993 Dec 31;75(7):1417-30. PMID:7916661
- ↑ 3.0 3.1 Riddle RD, Johnson RL, Laufer E, Tabin C. Sonic hedgehog mediates the polarizing activity of the ZPA. Cell. 1993 Dec 31;75(7):1401-16. PMID:8269518
- ↑ 4.0 4.1 Roelink H, Porter JA, Chiang C, Tanabe Y, Chang DT, Beachy PA, Jessell TM. Floor plate and motor neuron induction by different concentrations of the amino-terminal cleavage product of sonic hedgehog autoproteolysis. Cell. 1995 May 5;81(3):445-55. PMID:7736596
- ↑ Chuang PT, Kornberg TB. On the range of hedgehog signaling. Curr Opin Genet Dev. 2000 Oct;10(5):515-22. PMID:10980429
- ↑ 6.0 6.1 6.2 Pepinsky RB, Rayhorn P, Day ES, Dergay A, Williams KP, Galdes A, Taylor FR, Boriack-Sjodin PA, Garber EA. Mapping sonic hedgehog-receptor interactions by steric interference. J Biol Chem. 2000 Apr 14;275(15):10995-1001. PMID:10753901
- ↑ Pasca di Magliano M, Hebrok M. Hedgehog signalling in cancer formation and maintenance. Nat Rev Cancer. 2003 Dec;3(12):903-11. PMID:14737121 doi:10.1038/nrc1229
- ↑ 8.0 8.1 8.2 Ruiz i Altaba A, Sanchez P, Dahmane N. Gli and hedgehog in cancer: tumours, embryos and stem cells. Nat Rev Cancer. 2002 May;2(5):361-72. PMID:12044012 doi:10.1038/nrc796
- ↑ Roessler E, Belloni E, Gaudenz K, Jay P, Berta P, Scherer SW, Tsui LC, Muenke M. Mutations in the human Sonic Hedgehog gene cause holoprosencephaly. Nat Genet. 1996 Nov;14(3):357-60. PMID:8896572 doi:10.1038/ng1196-357
- ↑ 10.0 10.1 Bumcrot DA, Takada R, McMahon AP. Proteolytic processing yields two secreted forms of sonic hedgehog. Mol Cell Biol. 1995 Apr;15(4):2294-303. PMID:7891723
- ↑ Porter JA, Young KE, Beachy PA. Cholesterol modification of hedgehog signaling proteins in animal development. Science. 1996 Oct 11;274(5285):255-9. PMID:8824192
- ↑ Pepinsky RB, Zeng C, Wen D, Rayhorn P, Baker DP, Williams KP, Bixler SA, Ambrose CM, Garber EA, Miatkowski K, Taylor FR, Wang EA, Galdes A. Identification of a palmitic acid-modified form of human Sonic hedgehog. J Biol Chem. 1998 May 29;273(22):14037-45. PMID:9593755
- ↑ 13.0 13.1 13.2 13.3 13.4 13.5 13.6 Hall TM, Porter JA, Beachy PA, Leahy DJ. A potential catalytic site revealed by the 1.7-A crystal structure of the amino-terminal signalling domain of Sonic hedgehog. Nature. 1995 Nov 9;378(6553):212-6. PMID:7477329 doi:http://dx.doi.org/10.1038/378212a0
- ↑ 14.0 14.1 Bumcrot DA, McMahon AP. Sonic hedgehog: making the gradient. Chem Biol. 1996 Jan;3(1):13-6. PMID:8807822
- ↑ Placzek M, Jessell TM, Dodd J. Induction of floor plate differentiation by contact-dependent, homeogenetic signals. Development. 1993 Jan;117(1):205-18. PMID:8223247
- ↑ 16.0 16.1 16.2 Huangfu D, Anderson KV. Signaling from Smo to Ci/Gli: conservation and divergence of Hedgehog pathways from Drosophila to vertebrates. Development. 2006 Jan;133(1):3-14. PMID:16339192 doi:133/1/3
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