1gvg

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[[Image:1gvg.jpg|left|200px]]<br /><applet load="1gvg" size="450" color="white" frame="true" align="right" spinBox="true"
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[[Image:1gvg.jpg|left|200px]]<br /><applet load="1gvg" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1gvg, resolution 1.54&Aring;" />
caption="1gvg, resolution 1.54&Aring;" />
'''CRYSTAL STRUCTURE OF CLAVAMINATE SYNTHASE WITH NITRIC OXIDE'''<br />
'''CRYSTAL STRUCTURE OF CLAVAMINATE SYNTHASE WITH NITRIC OXIDE'''<br />
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==About this Structure==
==About this Structure==
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1GVG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus] with FE, SO4, NO, AKG and PCX as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Nmo Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GVG OCA].
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1GVG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus] with <scene name='pdbligand=FE:'>FE</scene>, <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=NO:'>NO</scene>, <scene name='pdbligand=AKG:'>AKG</scene> and <scene name='pdbligand=PCX:'>PCX</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Nmo+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GVG OCA].
==Reference==
==Reference==
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[[Category: trifunctional enzyme]]
[[Category: trifunctional enzyme]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 15:39:29 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:43:05 2008''

Revision as of 07:43, 3 February 2008

Image:1gvg.jpg

1gvg, resolution 1.54Å

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CRYSTAL STRUCTURE OF CLAVAMINATE SYNTHASE WITH NITRIC OXIDE

Overview

Clavaminate synthase (CAS), a 2-oxoglutarate (2OG) dependent dioxygenase, catalyses three steps in the biosynthesis of clavulanic acid. Crystals of, CAS complexed with Fe(II), 2OG and deoxyguanidinoproclavaminate were, exposed to nitric oxide (NO) acting as a dioxygen analogue. Prior to, exposure with NO, the active site Fe(II) is octahedrally coordinated by a, water molecule, the 2-oxo and 1-carboxylate groups of 2OG, and the, side-chains of an aspartyl and two histidinyl residues. NO binds to the, position previously occupied by the 2OG 1-carboxylate concomitant with, rearrangement of the latter to the position previously occupied by the, displaced water.

About this Structure

1GVG is a Single protein structure of sequence from Streptomyces clavuligerus with , , , and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.

Reference

Crystal structure of a clavaminate synthase-Fe(II)-2-oxoglutarate-substrate-NO complex: evidence for metal centered rearrangements., Zhang Z, Ren J, Harlos K, McKinnon CH, Clifton IJ, Schofield CJ, FEBS Lett. 2002 Apr 24;517(1-3):7-12. PMID:12062399

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