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1h95
From Proteopedia
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| - | [[Image:1h95.gif|left|200px]]<br /><applet load="1h95" size=" | + | [[Image:1h95.gif|left|200px]]<br /><applet load="1h95" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1h95" /> | caption="1h95" /> | ||
'''SOLUTION STRUCTURE OF THE SINGLE-STRANDED DNA-BINDING COLD SHOCK DOMAIN (CSD) OF HUMAN Y-BOX PROTEIN 1 (YB1) DETERMINED BY NMR (10 LOWEST ENERGY STRUCTURES)'''<br /> | '''SOLUTION STRUCTURE OF THE SINGLE-STRANDED DNA-BINDING COLD SHOCK DOMAIN (CSD) OF HUMAN Y-BOX PROTEIN 1 (YB1) DETERMINED BY NMR (10 LOWEST ENERGY STRUCTURES)'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1H95 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Known structural/functional Site: <scene name='pdbsite=DB:DNA-Binding Site, Identified By DNA-Titration Followed w ...'>DB</scene>. Full crystallographic information is available from [http:// | + | 1H95 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Known structural/functional Site: <scene name='pdbsite=DB:DNA-Binding+Site,+Identified+By+DNA-Titration+Followed+w+...'>DB</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H95 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: y-box]] | [[Category: y-box]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:47:50 2008'' |
Revision as of 07:47, 3 February 2008
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SOLUTION STRUCTURE OF THE SINGLE-STRANDED DNA-BINDING COLD SHOCK DOMAIN (CSD) OF HUMAN Y-BOX PROTEIN 1 (YB1) DETERMINED BY NMR (10 LOWEST ENERGY STRUCTURES)
Overview
The human Y-box protein 1 (YB-1) is a member of the Y-box protein family, a class of proteins involved in transcriptional and translational, regulation of a wide range of genes. Here, we report the solution, structure of the cold-shock domain (CSD) of YB-1, which is thought to be, responsible for nucleic acid binding. It is the first structure solved of, a eukaryotic member of the cold-shock protein family and consists of a, closed five-stranded anti-parallel beta-barrel capped by a long flexible, loop. The structure of CSD is similar to the OB-fold and a comparison with, bacterial cold-shock proteins shows that its structural properties are, conserved from bacteria to man. Our data suggest the presence of a, DNA-binding site consisting of a patch of positively charged and aromatic, residues on the surface of the beta-barrel. Further, it is shown that CSD, which has a preference for binding single-stranded pyrimidine-rich, sequences, binds weakly and hardly specifically to DNA. Binding affinities, reported for intact YB-1 indicate that domains other than the CSD play a, role in DNA binding of YB-1.
About this Structure
1H95 is a Single protein structure of sequence from Homo sapiens. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The solution structure and DNA-binding properties of the cold-shock domain of the human Y-box protein YB-1., Kloks CP, Spronk CA, Lasonder E, Hoffmann A, Vuister GW, Grzesiek S, Hilbers CW, J Mol Biol. 2002 Feb 15;316(2):317-26. PMID:11851341
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