1hoo
From Proteopedia
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[[Category: purine nucleotide biosynthesis]] | [[Category: purine nucleotide biosynthesis]] | ||
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Revision as of 13:33, 30 October 2007
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STRUCTURE OF GUANINE NUCLEOTIDE (GPPCP) COMPLEX OF ADENYLOSUCCINATE SYNTHETASE FROM E. COLI AT PH 6.5 AND 25 DEGREES CELSIUS
Overview
Structures of adenylosuccinate synthetase from Escherichia coli complexed, with guanosine-5'-(beta,gamma-imido) triphosphate and, guanosine-5'-(beta,gamma-methylene)triphosphate in the presence and the, absence of Mg2+ have been refined to R-factors below 0.2 against data to a, nominal resolution of 2.7 A. Asp333 of the synthetase hydrogen bonds to, the exocyclic 2-amino and endocyclic N1 groups of the guanine nucleotide, base, whereas the hydroxyl of Ser414 and the backbone amide of Lys331, hydrogen bond to the 6-oxo position. The side chains of Lys331 and Pro417, pack against opposite faces of the guanine nucleotide base. The synthetase, recognizes neither the N7 position of guanine nucleotides nor the ribose, group. Electron density for the guanine-5'-(beta,gamma-imido) triphosphate, ... [(full description)]
About this Structure
1HOO is a [Single protein] structure of sequence from [Escherichia coli] with GNH as [ligand]. Active as [Adenylosuccinate synthase], with EC number [6.3.4.4]. Structure known Active Sites: GNA and GNB. Full crystallographic information is available from [OCA].
Reference
Refined crystal structures of guanine nucleotide complexes of adenylosuccinate synthetase from Escherichia coli., Poland BW, Hou Z, Bruns C, Fromm HJ, Honzatko RB, J Biol Chem. 1996 Jun 28;271(26):15407-13. PMID:8663109
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