1hjk
From Proteopedia
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| - | [[Image:1hjk.gif|left|200px]]<br /><applet load="1hjk" size=" | + | [[Image:1hjk.gif|left|200px]]<br /><applet load="1hjk" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1hjk, resolution 2.3Å" /> | caption="1hjk, resolution 2.3Å" /> | ||
'''ALKALINE PHOSPHATASE MUTANT H331Q'''<br /> | '''ALKALINE PHOSPHATASE MUTANT H331Q'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1HJK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN, MG and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] Known structural/functional Sites: <scene name='pdbsite=CTA:Catalytically Active Site On A Subunit'>CTA</scene> and <scene name='pdbsite=CTB:Catalytically Active Site On B Subunit'>CTB</scene>. Full crystallographic information is available from [http:// | + | 1HJK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] Known structural/functional Sites: <scene name='pdbsite=CTA:Catalytically+Active+Site+On+A+Subunit'>CTA</scene> and <scene name='pdbsite=CTB:Catalytically+Active+Site+On+B+Subunit'>CTB</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJK OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transferase(phospho]] | [[Category: transferase(phospho]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:49:36 2008'' |
Revision as of 07:49, 3 February 2008
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ALKALINE PHOSPHATASE MUTANT H331Q
Overview
Using a mutant version of E. coli alkaline phosphatase, we succeeded in, trapping and determining the structure of the phospho-enzyme intermediate., The X-ray structure also revealed the catalytic water molecule, bound to, one of the active site zinc ions, positioned ideally for the apical attack, necessary for the hydrolysis of the intermediate.
About this Structure
1HJK is a Single protein structure of sequence from Escherichia coli with , and as ligands. Active as Alkaline phosphatase, with EC number 3.1.3.1 Known structural/functional Sites: and . Full crystallographic information is available from OCA.
Reference
Trapping and visualization of a covalent enzyme-phosphate intermediate., Murphy JE, Stec B, Ma L, Kantrowitz ER, Nat Struct Biol. 1997 Aug;4(8):618-22. PMID:9253408
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