1ikd
From Proteopedia
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| - | [[Image:1ikd.jpg|left|200px]]<br /><applet load="1ikd" size=" | + | [[Image:1ikd.jpg|left|200px]]<br /><applet load="1ikd" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ikd" /> | caption="1ikd" /> | ||
'''ACCEPTOR STEM, NMR, 30 STRUCTURES'''<br /> | '''ACCEPTOR STEM, NMR, 30 STRUCTURES'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1IKD is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Known structural/functional Site: <scene name='pdbsite=GU:Wobble Base-Pair'>GU</scene>. Full crystallographic information is available from [http:// | + | 1IKD is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Known structural/functional Site: <scene name='pdbsite=GU:Wobble+Base-Pair'>GU</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IKD OCA]. |
==Reference== | ==Reference== | ||
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[[Category: ribonucleic acid]] | [[Category: ribonucleic acid]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:50:37 2008'' |
Revision as of 07:50, 3 February 2008
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ACCEPTOR STEM, NMR, 30 STRUCTURES
Overview
The fidelity of translation of the genetic code depends on accurate tRNA, aminoacylation by cognate aminoacyl-tRNA synthetases. Thus, each tRNA has, specificity not only for codon recognition, but also for amino acid, identity; this aminoacylation specificity is referred to as tRNA identity., The primary determinant of the acceptor identity of Escherichia coli, tRNAAlais a wobble G3.U70 pair within the acceptor stem. Despite extensive, biochemical and genetic data, the mechanism by which the G3.U70 pair marks, the acceptor end of tRNAAla for aminoacylation with alanine has not been, clarified at the molecular level. The solution structure of a microhelix, derived from the tRNAAla acceptor end has been determined at high, precision using a very extensive set of experimental constraints, (approximately 32 per nt) obtained by heteronuclear multidimensional NMR, methods. The tRNAAla acceptor end is overall similar to A-form RNA, but, important differences are observed. The G3.U70 wobble pair distorts the, conformation of the phosphodiester backbone and presents the functional, groups of U70 in an unusual spatial location. The discriminator base A73, has extensive stacking overlap with G1 within the G1.C72 base pair at the, end of the double helical stem and the -CCA end is significantly less, ordered than the rest of the molecule.
About this Structure
1IKD is a Protein complex structure of sequences from [1]. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structure of the acceptor stem of Escherichia coli tRNA Ala: role of the G3.U70 base pair in synthetase recognition., Ramos A, Varani G, Nucleic Acids Res. 1997 Jun 1;25(11):2083-90. PMID:9153306
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