This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1o7j
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1o7j.jpg|left|200px]]<br /><applet load="1o7j" size=" | + | [[Image:1o7j.jpg|left|200px]]<br /><applet load="1o7j" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1o7j, resolution 1.0Å" /> | caption="1o7j, resolution 1.0Å" /> | ||
'''ATOMIC RESOLUTION STRUCTURE OF ERWINIA CHRYSANTHEMI L-ASPARAGINASE'''<br /> | '''ATOMIC RESOLUTION STRUCTURE OF ERWINIA CHRYSANTHEMI L-ASPARAGINASE'''<br /> | ||
| Line 7: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1O7J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi] with SO4, EDO and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Asparaginase Asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1] Known structural/functional Site: <scene name='pdbsite=AC1:Edo Binding Site For Chain D'>AC1</scene>. Full crystallographic information is available from [http:// | + | 1O7J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=EDO:'>EDO</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Asparaginase Asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1] Known structural/functional Site: <scene name='pdbsite=AC1:Edo+Binding+Site+For+Chain+D'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7J OCA]. |
==Reference== | ==Reference== | ||
| Line 26: | Line 26: | ||
[[Category: l-asparaginase]] | [[Category: l-asparaginase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:54:29 2008'' |
Revision as of 07:54, 3 February 2008
|
ATOMIC RESOLUTION STRUCTURE OF ERWINIA CHRYSANTHEMI L-ASPARAGINASE
Overview
An X-ray structure of L-asparaginase from Erwinia chrysanthemi (ErA) has, been refined at 1 A resolution to an R factor of below 0.1, using data, collected on a synchrotron source. With four molecules of the enzyme, consisting of 327 amino acids each, this crystal contains one of the, largest asymmetric units of a protein refined to date at atomic, resolution. Previously, structures of ErA and of related enzymes from, other bacterial sources have been refined at resolutions not exceeding 1.7, A; thus, the present structure represents a very significant improvement, in the quality of the available models of these proteins and should, provide a good basis for future studies of the conformational variability, of proteins, identification of subtle conformational features and, corroboration of the stereochemical libraries, amongst other things., L-Asparaginases, which are enzymes that catalyze the hydrolysis of, L-asparagine to aspartic acid, have been used for over 30 y as therapeutic, agents in the treatment of acute childhood lymphoblastic leukemia, although the details of the enzymatic reaction and substrate specificity, have not yet been completely elucidated. This atomic resolution structure, is a step in that direction.
About this Structure
1O7J is a Single protein structure of sequence from Erwinia chrysanthemi with , and as ligands. Active as Asparaginase, with EC number 3.5.1.1 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Atomic resolution structure of Erwinia chrysanthemi L-asparaginase., Lubkowski J, Dauter M, Aghaiypour K, Wlodawer A, Dauter Z, Acta Crystallogr D Biol Crystallogr. 2003 Jan;59(Pt 1):84-92. Epub 2002, Dec 19. PMID:12499544
Page seeded by OCA on Sun Feb 3 09:54:29 2008
Categories: Asparaginase | Erwinia chrysanthemi | Single protein | Aghaiypour, K. | Dauter, M. | Dauter, Z. | Lubkowski, J. | Wlodawer, A. | EDO | GOL | SO4 | Atomic resolution | Hydrolase | L-asparaginase
