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1ose
From Proteopedia
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| - | [[Image:1ose.jpg|left|200px]]<br /><applet load="1ose" size=" | + | [[Image:1ose.jpg|left|200px]]<br /><applet load="1ose" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ose, resolution 2.30Å" /> | caption="1ose, resolution 2.30Å" /> | ||
'''PORCINE PANCREATIC ALPHA-AMYLASE COMPLEXED WITH ACARBOSE'''<br /> | '''PORCINE PANCREATIC ALPHA-AMYLASE COMPLEXED WITH ACARBOSE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1OSE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with GLC, CL and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] Known structural/functional Sites: <scene name='pdbsite=AS1:Active Site, Sugar Binding Site'>AS1</scene>, <scene name='pdbsite=AS2:Active Site, Sugar Binding Site'>AS2</scene>, <scene name='pdbsite=AS3:Active Site, Sugar Binding Site'>AS3</scene>, <scene name='pdbsite=AS4:Active Site, Sugar Binding Site'>AS4</scene>, <scene name='pdbsite=AS5:Active Site, Sugar Binding Site'>AS5</scene>, <scene name='pdbsite=CAL:Ca Binding Site'>CAL</scene> and <scene name='pdbsite=CLO:Chloride Binding Site. There Are Six Bound Sugar Rings. ...'>CLO</scene>. Full crystallographic information is available from [http:// | + | 1OSE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=GLC:'>GLC</scene>, <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] Known structural/functional Sites: <scene name='pdbsite=AS1:Active+Site,+Sugar+Binding+Site'>AS1</scene>, <scene name='pdbsite=AS2:Active+Site,+Sugar+Binding+Site'>AS2</scene>, <scene name='pdbsite=AS3:Active+Site,+Sugar+Binding+Site'>AS3</scene>, <scene name='pdbsite=AS4:Active+Site,+Sugar+Binding+Site'>AS4</scene>, <scene name='pdbsite=AS5:Active+Site,+Sugar+Binding+Site'>AS5</scene>, <scene name='pdbsite=CAL:Ca+Binding+Site'>CAL</scene> and <scene name='pdbsite=CLO:Chloride+Binding+Site.+There+Are+Six+Bound+Sugar+Rings.+...'>CLO</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OSE OCA]. |
==Reference== | ==Reference== | ||
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[[Category: hydrolase (o-glycosyl)]] | [[Category: hydrolase (o-glycosyl)]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:59:44 2008'' |
Revision as of 07:59, 3 February 2008
|
PORCINE PANCREATIC ALPHA-AMYLASE COMPLEXED WITH ACARBOSE
Overview
Two different crystal forms of pig pancreatic alpha-amylase isoenzyme II, (PPAII), free and complexed to a carbohydrate inhibitor (acarbose), have, been compared together and to previously reported structures of PPAI. A, crystal form obtained at 4 degrees C, containing nearly 72% solvent, made, it possible to obtain a new complex with acarbose, different from a, previous one obtained at 20 degrees C [Qian, M., Buisson, G., Duee, E., Haser, H. & Payan, F. (1994) Biochemistry 33, 6284-6294]. In the present, form, six contiguous subsites of the enzyme active site are occupied by, the carbohydrate ligand; the structural data indicate that the binding, site is capable of holding more than the five glucose units of the scheme, proposed through kinetic studies. A monosaccharide ring bridging two, protein molecules related by the crystal packing is located on the, surface, at a distance of 2.0 nm from the reducing end of the inhibitor, ligand; the symmetry-related glucose ring in the crystal lattice is found, 1.5 nm away from the non-reducing end of the inhibitor ligand.
About this Structure
1OSE is a Single protein structure of sequence from Sus scrofa with , and as ligands. Active as Alpha-amylase, with EC number 3.2.1.1 Known structural/functional Sites: , , , , , and . Full crystallographic information is available from OCA.
Reference
Crystal structure of pig pancreatic alpha-amylase isoenzyme II, in complex with the carbohydrate inhibitor acarbose., Gilles C, Astier JP, Marchis-Mouren G, Cambillau C, Payan F, Eur J Biochem. 1996 Jun 1;238(2):561-9. PMID:8681972
Page seeded by OCA on Sun Feb 3 09:59:44 2008
Categories: Alpha-amylase | Single protein | Sus scrofa | Gilles, C. | Payan, F. | CA | CL | GLC | Acarbose | Hydrolase | Hydrolase (o-glycosyl)
