Molecular Playground/Human Galactosamine-6-sulfatase

From Proteopedia

Revision as of 20:00, 29 April 2010

One of the CBI Molecules being studied in the University of Massachusetts Amherst Chemistry-Biology Interface Program at UMass Amherst and on display at the Molecular Playground.

Human Lysosomal Protective Protein Cathepsin A (PPCA)

Human Lysosomal Protective Protein Cathepsin A

The human protective protein cathepsin A forms a multi-enzyme complex with beta-galactosidase and neuraminidase in lysosomes. PPCA has so far two functions:a catalytic activity overlapping with that of Cathepsin A and a protective function towards lysosomal beta-galactosidase and neuraminidase. The stability and activity of the latter glycosidases depend on their interaction with PPCA [1].

PPCA Glycosylation sites

PPCA, is synthesized as a 542 amino acid precursor with a molecular weight of 54kDa. and it dimerizes soon after synthesis. The sequence contains two glycosylation sites, Asn 117 and Asn 305. It also contains nine cysteines. The spinning protein ( ) is human protective protein with the glycosylation sites represented in stick model colored by atom.

References:

[1] Jackman, H. L., Morris, P. W., Deddish, P. A., Skidgel, R. A. and Erdos, E. G. (1992) J. Biol. Chem. 267, 2872-2875