1un1
From Proteopedia
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| - | [[Image:1un1.gif|left|200px]]<br /><applet load="1un1" size=" | + | [[Image:1un1.gif|left|200px]]<br /><applet load="1un1" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1un1, resolution 2.10Å" /> | caption="1un1, resolution 2.10Å" /> | ||
'''XYLOGLUCAN ENDOTRANSGLYCOSYLASE NATIVE STRUCTURE.'''<br /> | '''XYLOGLUCAN ENDOTRANSGLYCOSYLASE NATIVE STRUCTURE.'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1UN1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Populus_tremula Populus tremula] with AU as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Xyloglucan:xyloglucosyl_transferase Xyloglucan:xyloglucosyl transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.207 2.4.1.207] Known structural/functional Site: <scene name='pdbsite=AC1:Au Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http:// | + | 1UN1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Populus_tremula Populus tremula] with <scene name='pdbligand=AU:'>AU</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Xyloglucan:xyloglucosyl_transferase Xyloglucan:xyloglucosyl transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.207 2.4.1.207] Known structural/functional Site: <scene name='pdbsite=AC1:Au+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UN1 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: xyloglucan]] | [[Category: xyloglucan]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:02:55 2008'' |
Revision as of 08:02, 3 February 2008
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XYLOGLUCAN ENDOTRANSGLYCOSYLASE NATIVE STRUCTURE.
Overview
Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan, polymers in plant cell walls via a transglycosylation mechanism. Thus, XET, is a key enzyme in all plant processes that require cell wall remodeling., To provide a basis for detailed structure-function studies, the crystal, structure of Populus tremula x tremuloides XET16A (PttXET16A), heterologously expressed in Pichia pastoris, has been determined at 1.8-A, resolution. Even though the overall structure of PttXET16A is a curved, beta-sandwich similar to other enzymes in the glycoside hydrolase family, GH16, parts of its substrate binding cleft are more reminiscent of the, distantly related family GH7. In addition, XET has a C-terminal extension, that packs against the conserved core, providing an additional beta-strand, and a short alpha-helix. The structure of XET in complex with a xyloglucan, nonasaccharide, XLLG, reveals a very favorable acceptor binding site, which is a necessary but not sufficient prerequisite for, transglycosylation. Biochemical data imply that the enzyme requires sugar, residues in both acceptor and donor sites to properly orient the, glycosidic bond relative to the catalytic residues.
About this Structure
1UN1 is a Single protein structure of sequence from Populus tremula with as ligand. Active as Xyloglucan:xyloglucosyl transferase, with EC number 2.4.1.207 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Crystal structures of a poplar xyloglucan endotransglycosylase reveal details of transglycosylation acceptor binding., Johansson P, Brumer H 3rd, Baumann MJ, Kallas AM, Henriksson H, Denman SE, Teeri TT, Jones TA, Plant Cell. 2004 Apr;16(4):874-86. Epub 2004 Mar 12. PMID:15020748
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