1vhr
From Proteopedia
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| - | [[Image:1vhr.gif|left|200px]]<br /><applet load="1vhr" size=" | + | [[Image:1vhr.gif|left|200px]]<br /><applet load="1vhr" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1vhr, resolution 2.1Å" /> | caption="1vhr, resolution 2.1Å" /> | ||
'''HUMAN VH1-RELATED DUAL-SPECIFICITY PHOSPHATASE'''<br /> | '''HUMAN VH1-RELATED DUAL-SPECIFICITY PHOSPHATASE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1VHR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 and EPE as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] Known structural/functional Sites: <scene name='pdbsite=GAA:The Loop Containing The Putative General Acid ASP 92'>GAA</scene>, <scene name='pdbsite=GAB:The Loop Containing The Putative General Acid ASP 92'>GAB</scene>, <scene name='pdbsite=PLA:Phosphate Binding Loop Including Catalytic Nucleophile C ...'>PLA</scene>, <scene name='pdbsite=PLB:Phosphate Binding Loop Including Catalytic Nucleophile C ...'>PLB</scene>, <scene name='pdbsite=RCA:Designated Recognition Region In Primary Reference. Prop ...'>RCA</scene>, <scene name='pdbsite=RCB:Designated Recognition Region In Primary Reference. Prop ...'>RCB</scene>, <scene name='pdbsite=VRA:The Region Containing High Variation Among The Dual-Spec ...'>VRA</scene> and <scene name='pdbsite=VRB:The Region Containing High Variation Among The Dual-Spec ...'>VRB</scene>. Full crystallographic information is available from [http:// | + | 1VHR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=EPE:'>EPE</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] Known structural/functional Sites: <scene name='pdbsite=GAA:The+Loop+Containing+The+Putative+General+Acid+ASP+92'>GAA</scene>, <scene name='pdbsite=GAB:The+Loop+Containing+The+Putative+General+Acid+ASP+92'>GAB</scene>, <scene name='pdbsite=PLA:Phosphate+Binding+Loop+Including+Catalytic+Nucleophile+C+...'>PLA</scene>, <scene name='pdbsite=PLB:Phosphate+Binding+Loop+Including+Catalytic+Nucleophile+C+...'>PLB</scene>, <scene name='pdbsite=RCA:Designated+Recognition+Region+In+Primary+Reference.+Prop+...'>RCA</scene>, <scene name='pdbsite=RCB:Designated+Recognition+Region+In+Primary+Reference.+Prop+...'>RCB</scene>, <scene name='pdbsite=VRA:The+Region+Containing+High+Variation+Among+The+Dual-Spec+...'>VRA</scene> and <scene name='pdbsite=VRB:The+Region+Containing+High+Variation+Among+The+Dual-Spec+...'>VRB</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VHR OCA]. |
==Reference== | ==Reference== | ||
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[[Category: protein dual-specificity phosphatase]] | [[Category: protein dual-specificity phosphatase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:17:00 2008'' |
Revision as of 08:17, 3 February 2008
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HUMAN VH1-RELATED DUAL-SPECIFICITY PHOSPHATASE
Overview
Dual specificity protein phosphatases (DSPs) regulate mitogenic signal, transduction and control the cell cycle. Here, the crystal structure of a, human DSP, vaccinia H1-related phosphatase (or VHR), was determined at 2.1, angstrom resolution. A shallow active site pocket in VHR allows for the, hydrolysis of phosphorylated serine, threonine, or tyrosine protein, residues, whereas the deeper active site of protein tyrosine phosphatases, (PTPs) restricts substrate specificity to only phosphotyrosine. Positively, charged crevices near the active site may explain the enzyme's preference, for substrates with two phosphorylated residues. The VHR structure defines, a conserved structural scaffold for both DSPs and PTPs. A "recognition, region," connecting helix alpha1 to strand beta1, may determine, differences in substrate specificity between VHR, the PTPs, and other, DSPs.
About this Structure
1VHR is a Single protein structure of sequence from Homo sapiens with and as ligands. Active as Protein-tyrosine-phosphatase, with EC number 3.1.3.48 Known structural/functional Sites: , , , , , , and . Full crystallographic information is available from OCA.
Reference
Crystal structure of the dual specificity protein phosphatase VHR., Yuvaniyama J, Denu JM, Dixon JE, Saper MA, Science. 1996 May 31;272(5266):1328-31. PMID:8650541
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