1w0k
From Proteopedia
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| - | [[Image:1w0k.gif|left|200px]]<br /><applet load="1w0k" size=" | + | [[Image:1w0k.gif|left|200px]]<br /><applet load="1w0k" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1w0k, resolution 2.85Å" /> | caption="1w0k, resolution 2.85Å" /> | ||
'''BERYLLIUM FLUORIDE INHIBITED BOVINE F1-ATPASE'''<br /> | '''BERYLLIUM FLUORIDE INHIBITED BOVINE F1-ATPASE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1W0K is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with MG, PO4, ADP and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14] Known structural/functional Site: <scene name='pdbsite=PLP:Mg Binding Site For Chain F'>PLP</scene>. Full crystallographic information is available from [http:// | + | 1W0K is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=PO4:'>PO4</scene>, <scene name='pdbligand=ADP:'>ADP</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14] Known structural/functional Site: <scene name='pdbsite=PLP:Mg+Binding+Site+For+Chain+F'>PLP</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W0K OCA]. |
==Reference== | ==Reference== | ||
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[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:17:50 2008'' |
Revision as of 08:17, 3 February 2008
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BERYLLIUM FLUORIDE INHIBITED BOVINE F1-ATPASE
Overview
The structure of bovine F1-ATPase inhibited with ADP and beryllium, fluoride at 2.0 angstroms resolution contains two ADP.BeF3- complexes, mimicking ATP, bound in the catalytic sites of the beta(TP) and beta(DP), subunits. Except for a 1 angstrom shift in the guanidinium of alphaArg373, the conformations of catalytic side chains are very similar in both sites., However, the ordered water molecule that carries out nucleophilic attack, on the gamma-phosphate of ATP during hydrolysis is 2.6 angstroms from the, beryllium in the beta(DP) subunit and 3.8 angstroms away in the beta(TP), subunit, strongly indicating that the beta(DP) subunit is the, catalytically active conformation. In the structure of F1-ATPase with five, bound ADP molecules (three in alpha-subunits, one each in the beta(TP) and, beta(DP) subunits), which has also been determined, the conformation of, alphaArg373 suggests that it senses the presence (or absence) of the, gamma-phosphate of ATP. Two catalytic schemes are discussed concerning the, various structures of bovine F1-ATPase.
About this Structure
1W0K is a Protein complex structure of sequences from Bos taurus with , , and as ligands. Active as H(+)-transporting two-sector ATPase, with EC number 3.6.3.14 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The structure of bovine F1-ATPase inhibited by ADP and beryllium fluoride., Kagawa R, Montgomery MG, Braig K, Leslie AG, Walker JE, EMBO J. 2004 Jul 21;23(14):2734-44. Epub 2004 Jul 1. PMID:15229653
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Categories: Bos taurus | H(+)-transporting two-sector ATPase | Protein complex | Braig, K. | Kagawa, R. | Leslie, A.G.W. | Montgomery, M.G. | Walker, J.E. | ADP | GOL | MG | PO4 | Atp phosphorylase | Atp phosphorylase (h+ transporting) | Atp synthase | Atp synthesis | Atp-binding | F1-atpase | F1fo atp synthase | Hydrolase
