1w72
From Proteopedia
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| - | [[Image:1w72.gif|left|200px]]<br /><applet load="1w72" size=" | + | [[Image:1w72.gif|left|200px]]<br /><applet load="1w72" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1w72, resolution 2.15Å" /> | caption="1w72, resolution 2.15Å" /> | ||
'''CRYSTAL STRUCTURE OF HLA-A1:MAGE-A1 IN COMPLEX WITH FAB-HYB3'''<br /> | '''CRYSTAL STRUCTURE OF HLA-A1:MAGE-A1 IN COMPLEX WITH FAB-HYB3'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1W72 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with GOL as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Gol Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http:// | + | 1W72 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Gol+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W72 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: tcr-like binding]] | [[Category: tcr-like binding]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:20:06 2008'' |
Revision as of 08:20, 3 February 2008
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CRYSTAL STRUCTURE OF HLA-A1:MAGE-A1 IN COMPLEX WITH FAB-HYB3
Contents |
Overview
Antibodies with T cell receptor-like specificity possess a considerable, diagnostic and therapeutic potential, but the structural basis of the, interaction between an antibody and an histocompatibility antigen has so, far not been determined. We present here the crystal structure (at 2.15 A, resolution) of the recombinant, affinity-matured human antibody fragment, Fab-Hyb3 bound to the tumor-associated human leukocyte antigen, (HLA)/peptide complex HLA-A1.MAGE-A1. Fab-Hyb3 employs a diagonal docking, mode resembling that of T cell receptors. However, other than these, natural ligands, the antibody uses only four of its six, complementarity-determining regions for direct interactions with the, target. It recognizes the C-terminal half of the MAGE-A1 peptide, the, HLA-A1 alpha1-helix, and N-terminal residues of the alpha2-helix, accompanied by a large tilting angle between the two types of molecules, within the complex. Interestingly, only a single hydrogen bond between a, peptide side chain and Fab-Hyb3 contributes to the interaction, but large, buried surface areas with pronounced shape complementarity assure high, affinity and specificity for MAGE-A1. The HLA-A1.MAGE-A1.antibody, structure is discussed in comparison with those of natural ligands, recognizing HLA.peptide complexes.
Disease
Known diseases associated with this structure: Abacavir hypersensitivity, susceptibility to OMIM:[142800], Ankylosing spondylitis, susceptibility to, 1 OMIM:[142800], Stevens-Johnson syndrome, susceptibility to OMIM:[142800]
About this Structure
1W72 is a Protein complex structure of sequences from Homo sapiens with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
A major histocompatibility complex-peptide-restricted antibody and t cell receptor molecules recognize their target by distinct binding modes: crystal structure of human leukocyte antigen (HLA)-A1-MAGE-A1 in complex with FAB-HYB3., Hulsmeyer M, Chames P, Hillig RC, Stanfield RL, Held G, Coulie PG, Alings C, Wille G, Saenger W, Uchanska-Ziegler B, Hoogenboom HR, Ziegler A, J Biol Chem. 2005 Jan 28;280(4):2972-80. Epub 2004 Nov 10. PMID:15537658
Page seeded by OCA on Sun Feb 3 10:20:06 2008
Categories: Homo sapiens | Protein complex | Alings, C. | Chames, P. | Coulie, P.G. | Held, G. | Hillig, R.C. | Hoogenboom, H.R. | Hulsmeyer, M. | Saenger, W. | Stanfield, R.L. | Uchanska-Ziegler, B. | Wille, G. | Ziegler, A. | GOL | Hla | Human leucocyte antigen | Mage | Mhc-i | Peptide-specific fab | Tcr-like binding
