1w9d
From Proteopedia
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| - | [[Image:1w9d.gif|left|200px]]<br /><applet load="1w9d" size=" | + | [[Image:1w9d.gif|left|200px]]<br /><applet load="1w9d" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1w9d, resolution 1.6Å" /> | caption="1w9d, resolution 1.6Å" /> | ||
'''S. ALBA MYROSINASE IN COMPLEX WITH S-ETHYL PHENYLACETOTHIOHYDROXIMATE-O-SULFATE'''<br /> | '''S. ALBA MYROSINASE IN COMPLEX WITH S-ETHYL PHENYLACETOTHIOHYDROXIMATE-O-SULFATE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1W9D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sinapis_alba Sinapis alba] with NAG, ZN, SO4, SEH and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Transferred_entry:_3.2.1.147 Transferred entry: 3.2.1.147], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.3.1 3.2.3.1] Known structural/functional Site: <scene name='pdbsite=AGB:Gol Binding Site For Chain M'>AGB</scene>. Full crystallographic information is available from [http:// | + | 1W9D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sinapis_alba Sinapis alba] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=SEH:'>SEH</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Transferred_entry:_3.2.1.147 Transferred entry: 3.2.1.147], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.3.1 3.2.3.1] Known structural/functional Site: <scene name='pdbsite=AGB:Gol+Binding+Site+For+Chain+M'>AGB</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W9D OCA]. |
==Reference== | ==Reference== | ||
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[[Category: thiohydroximate]] | [[Category: thiohydroximate]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:20:48 2008'' |
Revision as of 08:20, 3 February 2008
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S. ALBA MYROSINASE IN COMPLEX WITH S-ETHYL PHENYLACETOTHIOHYDROXIMATE-O-SULFATE
Overview
Myrosinase, a thioglucoside glucohydrolase, is the only enzyme able to, hydrolyse glucosinolates, a unique family of molecules bearing an anomeric, O-sulfated thiohydroximate function. Non-hydrolysable myrosinase, inhibitors have been devised and studied for their biological interaction., Diverse modifications of the O-sulfate moiety did not result in a, significant inhibitory effect, whereas replacing the D-glucopyrano residue, by its carba-analogue allowed inhibition to take place. X-Ray experiments, carried out after soaking allowed for the first time inclusion of a, non-hydrolysable inhibitor inside the enzymatic pocket. Structural tuning, of the aglycon part in its pocket is being used as a guide for the, development of simplified and more potent inhibitors.
About this Structure
1W9D is a Single protein structure of sequence from Sinapis alba with , , , and as ligands. Active as Transferred entry: 3.2.1.147, with EC number 3.2.3.1 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The glucosinolate-myrosinase system. New insights into enzyme-substrate interactions by use of simplified inhibitors., Bourderioux A, Lefoix M, Gueyrard D, Tatibouet A, Cottaz S, Arzt S, Burmeister WP, Rollin P, Org Biomol Chem. 2005 May 21;3(10):1872-9. Epub 2005 Apr 14. PMID:15889170
Page seeded by OCA on Sun Feb 3 10:20:48 2008
Categories: Sinapis alba | Single protein | Transferred entry: 3.2.1.147 | Arzt, S. | Bourderioux, A. | Burmeister, W.P. | Cottaz, S. | Gueyrard, D. | Lefoix, M. | Rollin, P. | Tatibouet, A. | GOL | NAG | SEH | SO4 | ZN | Glucotropaeolin | Glusosinolate | Hydrolase | Myrosinase | Thio-glucoside | Thioglucosidase | Thioglycosidase | Thiohydroximate
