2c81
From Proteopedia
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| - | [[Image:2c81.gif|left|200px]]<br /><applet load="2c81" size=" | + | [[Image:2c81.gif|left|200px]]<br /><applet load="2c81" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2c81, resolution 1.70Å" /> | caption="2c81, resolution 1.70Å" /> | ||
'''CRYSTAL STRUCTURES OF THE PLP- AND PMP-BOUND FORMS OF BTRR, A DUAL FUNCTIONAL AMINOTRANSFERASE INVOLVED IN BUTIROSIN BIOSYNTHESIS.'''<br /> | '''CRYSTAL STRUCTURES OF THE PLP- AND PMP-BOUND FORMS OF BTRR, A DUAL FUNCTIONAL AMINOTRANSFERASE INVOLVED IN BUTIROSIN BIOSYNTHESIS.'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2C81 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans] with PMP as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Pmp Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http:// | + | 2C81 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans] with <scene name='pdbligand=PMP:'>PMP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Pmp+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C81 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:32:36 2008'' |
Revision as of 08:32, 3 February 2008
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CRYSTAL STRUCTURES OF THE PLP- AND PMP-BOUND FORMS OF BTRR, A DUAL FUNCTIONAL AMINOTRANSFERASE INVOLVED IN BUTIROSIN BIOSYNTHESIS.
Overview
The aminotransferase (BtrR), which is involved in the biosynthesis of, butirosin, a 2-deoxystreptamine (2-DOS)-containing aminoglycoside, antibiotic produced by Bacillus circulans, catalyses the pyridoxal, phosphate (PLP)-dependent transamination reaction both of, 2-deoxy-scyllo-inosose to 2-deoxy-scyllo-inosamine and of, amino-dideoxy-scyllo-inosose to 2-DOS. The high-resolution crystal, structures of the PLP- and PMP-bound forms of BtrR aminotransferase from, B. circulans were solved at resolutions of 2.1 A and 1.7 A with, R(factor)/R(free) values of 17.4/20.6 and 19.9/21.9, respectively. BtrR, has a fold characteristic of the aspartate aminotransferase family, and, sequence and structure analysis categorises it as a member of SMAT, (secondary metabolite aminotransferases) subfamily. It exists as a, homodimer with two active sites per dimer. The active site of the BtrR, protomer is located in a cleft between an alpha helical N-terminus, a, central alphabetaalpha sandwich domain and an alphabeta C-terminal domain., The structures of the PLP- and PMP-bound enzymes are very similar; however, BtrR-PMP lacks the covalent bond to Lys192. Furthermore, the two forms, differ in the side-chain conformations of Trp92, Asp163, and Tyr342 that, are likely to be important in substrate selectivity and substrate binding., This is the first three-dimensional structure of an enzyme from the, butirosin biosynthesis gene cluster.
About this Structure
2C81 is a Single protein structure of sequence from Bacillus circulans with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Crystal structures of the PLP- and PMP-bound forms of BtrR, a dual functional aminotransferase involved in butirosin biosynthesis., Popovic B, Tang X, Chirgadze DY, Huang F, Blundell TL, Spencer JB, Proteins. 2006 Oct 1;65(1):220-30. PMID:16894611
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