2fua
From Proteopedia
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| - | [[Image:2fua.jpg|left|200px]]<br /><applet load="2fua" size=" | + | [[Image:2fua.jpg|left|200px]]<br /><applet load="2fua" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2fua, resolution 2.0Å" /> | caption="2fua, resolution 2.0Å" /> | ||
'''L-FUCULOSE 1-PHOSPHATE ALDOLASE CRYSTAL FORM T WITH COBALT'''<br /> | '''L-FUCULOSE 1-PHOSPHATE ALDOLASE CRYSTAL FORM T WITH COBALT'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2FUA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with CO, SO4 and BME as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/L-fuculose-phosphate_aldolase L-fuculose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.17 4.1.2.17] Known structural/functional Sites: <scene name='pdbsite=ACT:The Active Site Is Defined By The Co Ion And The Four Co ...'>ACT</scene> and <scene name='pdbsite=PBS:The Phosphate Binding Site Near The Active Center Is Occ ...'>PBS</scene>. Full crystallographic information is available from [http:// | + | 2FUA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=CO:'>CO</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=BME:'>BME</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/L-fuculose-phosphate_aldolase L-fuculose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.17 4.1.2.17] Known structural/functional Sites: <scene name='pdbsite=ACT:The+Active+Site+Is+Defined+By+The+Co+Ion+And+The+Four+Co+...'>ACT</scene> and <scene name='pdbsite=PBS:The+Phosphate+Binding+Site+Near+The+Active+Center+Is+Occ+...'>PBS</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FUA OCA]. |
==Reference== | ==Reference== | ||
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[[Category: lyase (aldehyde)]] | [[Category: lyase (aldehyde)]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:37:51 2008'' |
Revision as of 08:37, 3 February 2008
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L-FUCULOSE 1-PHOSPHATE ALDOLASE CRYSTAL FORM T WITH COBALT
Overview
The structure of the class II zinc-ion dependent L-fuculose-1-phosphate, aldolase from Escherichia coli in its tetragonal crystal form has been, established at 1.92 A resolution. The homotetrameric enzyme has a, molecular mass of 4 x 24 kDa and follows C(4) symmetry. The structure, model is exactly symmetrical, which contradicts an observed birefringence, anomaly of the crystals. The four catalytic centers are located in deep, clefts at the interfaces of adjacent subunits. The zinc ion is coordinated, by three histidines and one glutamate in an almost tetrahedral, arrangement. In contrast to numerous other catalytically competent zinc, ions, there is no water molecule in the ligand sphere. Replacement of zinc, by a cobalt ion caused only small structural changes. A search through the, Protein Data Bank indicated that the chain fold is novel. Sequence, homology searches revealed a significant similarity to the bacterial, L-ribulose-5-phosphate 4-epimerase.
About this Structure
2FUA is a Single protein structure of sequence from Escherichia coli with , and as ligands. Active as L-fuculose-phosphate aldolase, with EC number 4.1.2.17 Known structural/functional Sites: and . Full crystallographic information is available from OCA.
Reference
Refined high-resolution structure of the metal-ion dependent L-fuculose-1-phosphate aldolase (class II) from Escherichia coli., Dreyer MK, Schulz GE, Acta Crystallogr D Biol Crystallogr. 1996 Nov 1;52(Pt 6):1082-91. PMID:15299567
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