2j0t
From Proteopedia
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| - | [[Image:2j0t.jpg|left|200px]]<br /><applet load="2j0t" size=" | + | [[Image:2j0t.jpg|left|200px]]<br /><applet load="2j0t" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2j0t, resolution 2.54Å" /> | caption="2j0t, resolution 2.54Å" /> | ||
'''CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF MMP-1 IN COMPLEX WITH THE INHIBITORY DOMAIN OF TIMP-1'''<br /> | '''CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF MMP-1 IN COMPLEX WITH THE INHIBITORY DOMAIN OF TIMP-1'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2J0T is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Interstitial_collagenase Interstitial collagenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.7 3.4.24.7] Known structural/functional Site: <scene name='pdbsite=AC1:Ca Binding Site For Chain C'>AC1</scene>. Full crystallographic information is available from [http:// | + | 2J0T is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Interstitial_collagenase Interstitial collagenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.7 3.4.24.7] Known structural/functional Site: <scene name='pdbsite=AC1:Ca+Binding+Site+For+Chain+C'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J0T OCA]. |
==Reference== | ==Reference== | ||
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[[Category: zymogen]] | [[Category: zymogen]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:40:21 2008'' |
Revision as of 08:40, 3 February 2008
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CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF MMP-1 IN COMPLEX WITH THE INHIBITORY DOMAIN OF TIMP-1
Contents |
Overview
The mammalian collagenases are a subgroup of the matrix metalloproteinases, (MMPs) that are uniquely able to cleave triple helical fibrillar, collagens. Collagen breakdown is an essential part of extracellular matrix, turnover in key physiological processes including morphogenesis and wound, healing; however, unregulated collagenolysis is linked to important, diseases such as arthritis and cancer. The tissue inhibitors of, metalloproteinases (TIMPs) function in controlling the activity of MMPs, including collagenases. We report here the structure of a complex of the, catalytic domain of fibroblast collagenase (MMP-1) with the N-terminal, inhibitory domain of human TIMP-1 (N-TIMP-1) at 2.54 A resolution., Comparison with the previously reported structure of the, TIMP-1/stromelysin-1 (MMP-3) complex shows that the mechanisms of, inhibition of both MMPs are generally similar, yet there are significant, differences in the protein-protein interfaces in the two complexes., Specifically, the loop between beta-strands A and B of TIMP-1 makes, contact with MMP-3 but not with MMP-1, and there are marked differences in, the roles of individual residues in the C-D connector of TIMP-1 in binding, to the two MMPs. Structural rearrangements in the bound MMPs are also, strikingly different. This is the first crystallographic structure that, contains the truncated N-terminal domain of a TIMP, which shows only minor, differences from the corresponding region of the full-length protein., Differences in the interactions in the two TIMP-1 complexes provide a, structural explanation for the results of previous mutational studies and, a basis for designing new N-TIMP-1 variants with restricted specificity.
Disease
Known diseases associated with this structure: COPD, rate of decline of lung function in OMIM:[120353], Sorsby fundus dystrophy OMIM:[188826]
About this Structure
2J0T is a Protein complex structure of sequences from Homo sapiens with and as ligands. Active as Interstitial collagenase, with EC number 3.4.24.7 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Crystal structure of the catalytic domain of matrix metalloproteinase-1 in complex with the inhibitory domain of tissue inhibitor of metalloproteinase-1., Iyer S, Wei S, Brew K, Acharya KR, J Biol Chem. 2007 Jan 5;282(1):364-71. Epub 2006 Oct 18. PMID:17050530
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Categories: Homo sapiens | Interstitial collagenase | Protein complex | Acharya, K.R. | Brew, K. | Iyer, S. | Wei, S. | CA | ZN | Autocatalytic cleavage | Calcium | Collagen degradation | Collagenase | Erythrocyte maturation | Extracellular matrix | Glycoprotein | Hydrolase | Matrix metalloprotease | Metal-binding | Metalloenzyme inhibitor | Metalloprotease | Metalloprotease inhibitor | Ob fold | Polymorphism | Protease | Tissue inhibitor of metalloproteinase | Zinc | Zymogen
