2j1p
From Proteopedia
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| - | [[Image:2j1p.jpg|left|200px]]<br /><applet load="2j1p" size=" | + | [[Image:2j1p.jpg|left|200px]]<br /><applet load="2j1p" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2j1p, resolution 1.80Å" /> | caption="2j1p, resolution 1.80Å" /> | ||
'''GERANYLGERANYL DIPHOSPHATE SYNTHASE FROM SINAPIS ALBA IN COMPLEX WITH GGPP'''<br /> | '''GERANYLGERANYL DIPHOSPHATE SYNTHASE FROM SINAPIS ALBA IN COMPLEX WITH GGPP'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2J1P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sinapis_alba Sinapis alba] with GRG, PGO and BME as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Bme Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http:// | + | 2J1P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sinapis_alba Sinapis alba] with <scene name='pdbligand=GRG:'>GRG</scene>, <scene name='pdbligand=PGO:'>PGO</scene> and <scene name='pdbligand=BME:'>BME</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Bme+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J1P OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transit peptide]] | [[Category: transit peptide]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:40:39 2008'' |
Revision as of 08:40, 3 February 2008
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GERANYLGERANYL DIPHOSPHATE SYNTHASE FROM SINAPIS ALBA IN COMPLEX WITH GGPP
Overview
The crystal structure of the geranylgeranyl diphosphate synthase from, Sinapis alba (mustard) has been solved in two crystal forms at 1.8 and 2.0, A resolutions. In one of these forms, the dimeric enzyme binds one, molecule of the final product geranylgeranyl diphosphate in one subunit., The chainfold of the enzyme corresponds to that of other members of the, farnesyl diphosphate synthase family. Whereas the binding modes of the two, substrates dimethylallyl diphosphate and isopentenyl diphosphate at the, allyl and isopentenyl sites, respectively, have been established with, other members of the family, the complex structure presented reveals for, the first time the binding mode of a reaction product at the isopentenyl, site. The binding geometry of substrates and product in conjunction with, the protein environment and the established chemistry of the reaction, provide a clear picture of the reaction steps and atom displacements., Moreover, a comparison with a ligated homologous structure outlined an, appreciable induced fit: helix alpha8 and its environment undergo a large, conformational change when either the substrate dimethylallyl diphosphate, or an analogue is bound to the allyl site; only a minor conformational, change occurs when the other substrate isopentenyl diphosphate or the, product is bound to the isopentenyl site.
About this Structure
2J1P is a Single protein structure of sequence from Sinapis alba with , and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structure and Reaction Geometry of Geranylgeranyl Diphosphate Synthase from Sinapis alba(,)., Kloer DP, Welsch R, Beyer P, Schulz GE, Biochemistry. 2006 Dec 26;45(51):15197-204. Epub 2006 Dec 1. PMID:17176041
Page seeded by OCA on Sun Feb 3 10:40:39 2008
Categories: Sinapis alba | Single protein | Beyer, P. | Kloer, D.P. | Schulz, G.E. | Welsch, R. | BME | GRG | PGO | Carotenoid biosynthesis | Chloroplast | Geranylgeranyl diphosphate | Isoprene biosynthesis | Isoprenoid diphosphate synthase | Isoprenyl transtransferase | Multifunctional enzyme | Plastid | Transferase | Transit peptide
